一种基于重组包膜蛋白的酶联免疫吸附测定法用于西尼罗河病毒血清学诊断。
A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis.
作者信息
Wang Tian, Magnarelli Louis A, Anderson John F, Gould L Hannah, Bushmich Sandra L, Wong Susan J, Fikrig Erol
机构信息
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
出版信息
Vector Borne Zoonotic Dis. 2002 Summer;2(2):105-9. doi: 10.1089/153036602321131904.
Recombinant West Nile virus envelope (E) protein was examined in enzyme-linked immunosorbent assay (ELISA) to detect antibodies elicited during West Nile virus infection. Horses (nine of 10) and humans (six of six) with confirmed West Nile virus infection had IgG and/or IgM antibodies to the E protein. Antibodies to the recombinant West Nile virus membrane and nonstructural 1 proteins were not detected in any of these sera. An E protein-based ELISA may aid in the serological diagnosis of West Nile virus infection.
采用酶联免疫吸附测定（ELISA）检测重组西尼罗河病毒包膜（E）蛋白，以检测西尼罗河病毒感染期间产生的抗体。确诊为西尼罗河病毒感染的马匹（10匹中的9匹）和人类（6人中的6人）具有针对E蛋白的IgG和/或IgM抗体。在这些血清中均未检测到针对重组西尼罗河病毒膜蛋白和非结构1蛋白的抗体。基于E蛋白的ELISA可能有助于西尼罗河病毒感染的血清学诊断。
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