Bedirli Abdulkadir, Gokahmetoglu Selma, Sakrak Omer, Ersoz Nail, Ayangil Demet, Esin Hüseyin
Department of General Surgery, University of Erciyes, School of Medicine, 38039, Kayseri, Turkey.
Am J Surg. 2003 Apr;185(4):339-43. doi: 10.1016/s0002-9610(02)01418-6.
To investigate the effects of beta-glucan on intraabdominal abscess and adhesion formation after ileocolic anastomosis in a rat bacterial peritonitis model.
Sixty male Wistar rats were used in this study. Bacterial peritonitis was induced by performing a cecal ligation and puncture (CLP). On the first day, abdomen was reopened and peritoneal fluid samples were taken for microbiological examination. Thereafter, cecum was resected and ileocolic anastomosis was made. Group 1 rats were given 1 mL of normal saline as a placebo. Group 2 and group 3 rats were given beta-D-glucan 2 mg/kg by intramuscularly; 1 mg of beta-1,3-D-glucanase was administered to group 3 rats just after the use of beta-D-glucan. Half of each group were killed at day 7 and at day 21, respectively. Adhesions were scored and the presence of intraabdominal abscesses was noted.
One day after CLP, microbiological examination showed polymicrobial bacterial peritonitis. Five (8%) of the 60 animals died owing to sepsis. One week after CLP, in two rats in each group developed abscess formation. Three weeks after CLP, abscess formation was observed in only one rat in each group. The rats treated with the beta-glucan had significantly lower adhesion scores than did the saline-treated rats (P = 0.008 at one week; P = 0.001 at 3 weeks). Administration of beta-glucanase inhibited beta-glucan activity and resulted in more adhesions (P = 0.022 at 1 week; P = 0.006 at 3 weeks).
Although the use of beta-glucan after ileocolic anastomosis in rats with experimentally developed intraabdominal sepsis does not have any significantly effect on mortality and abscess formation, beta-glucan is capable of reducing the frequency of adhesion. This effect of beta-glucan has been prevented with beta-glucanase
在大鼠细菌性腹膜炎模型中，研究β-葡聚糖对回结肠吻合术后腹腔脓肿及粘连形成的影响。
本研究使用60只雄性Wistar大鼠。通过盲肠结扎和穿刺（CLP）诱导细菌性腹膜炎。第一天，打开腹腔并采集腹腔液样本进行微生物学检查。此后，切除盲肠并进行回结肠吻合术。第1组大鼠给予1 mL生理盐水作为安慰剂。第2组和第3组大鼠肌肉注射2 mg/kg的β-D-葡聚糖；第3组大鼠在使用β-D-葡聚糖后立即给予1 mg的β-1,3-D-葡聚糖酶。每组各有一半分别在第7天和第21天处死。对粘连进行评分并记录腹腔脓肿的存在情况。
CLP术后一天，微生物学检查显示为多菌种细菌性腹膜炎。60只动物中有5只（8%）因败血症死亡。CLP术后一周，每组有两只大鼠形成脓肿。CLP术后三周，每组仅一只大鼠观察到脓肿形成。用β-葡聚糖治疗的大鼠粘连评分显著低于生理盐水治疗的大鼠（一周时P = 0.008；三周时P = 0.001）。给予β-葡聚糖酶抑制了β-葡聚糖的活性并导致更多粘连（一周时P = 0.022；三周时P = 0.006）。
尽管在实验性腹腔脓毒症大鼠中，回结肠吻合术后使用β-葡聚糖对死亡率和脓肿形成没有显著影响，但β-葡聚糖能够降低粘连的发生率。β-葡聚糖酶可阻止β-葡聚糖的这种作用。
