非汞巯基试剂对大鼠肝脏胆甾-5-烯-3β-醇（胆固醇）生物合成的体外抑制作用。
In vitro inhibition of rat liver cholest-5en-3 beta-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents.
作者信息
Abernethy D, Hignite C, Azarnoff D L
出版信息
Steroids. 1976 Mar;27(3):297-307. doi: 10.1016/0039-128x(76)90052-0.
In vitro conversion of 2-14C-mevalonate to cholest-5en-3 beta-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, beta-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5alpha-cholest-8en-3beta-ol and the other tentatively identified as 4,4 dimethyl-5alpha-cholest-8,24-dien-3beta-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5alpha-lanosta-8,24-dien-3beta-ol 14alpha demethylase.
在大鼠肝脏匀浆中，亚砷酸盐、β-巯基乙醇、二硫苏糖醇和乙硫醇可抑制2-¹⁴C-甲羟戊酸在体外转化为胆甾-5-烯-3β-醇（胆固醇）。在这些条件下，两种含20个碳原子的甾醇会积累。其中一种被鉴定为4,4-二甲基-5α-胆甾-8-烯-3β-醇，另一种暂定为4,4-二甲基-5α-胆甾-8,24-二烯-3β-醇。基于这些观察结果，这些非汞巯基试剂不会抑制5α-羊毛甾-8,24-二烯-3β-醇14α-脱甲基酶。
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