Deng Xiaorong, Yang Zhen
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
J Huazhong Univ Sci Technolog Med Sci. 2002;22(1):37-41. doi: 10.1007/BF02904784.
The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (Pp), portal blood flow quantity (Qp) and ETA and ETB receptor mRNA expression in human cirrhosis. In situ hybridization and reverse-transcription polymerase chain reactions (RT-PCR) were performed to determined the expression of ETA and ETB receptor mRNA in liver tissues from traumatic subjects (n = 10) and cirrhotic patients (n = 15) in whom hepatic hemodynamic values were measured. The expression of the two transcripts was significantly higher in liver samples of cirrhotic patients than in those obtained from traumatic subjects. It has shown that ETA receptor mRNA predominantly located in hepatic stellate cells (HSCs) and vascular smooth muscle cells of intrahepatic arteries and portal veins, ETB receptor mRNA in HSCs, sinusoidal endothelial cells and Kuppfer cells. There was a highly significant direct relationship between ETA and ETB receptor mRNA and Pp and Qp in cirrhotic patients. It suggests that liver paracrine endothelin system may be overactivated in human cirrhosis accompanied with increased expression of ETA and ETB receptor mRNA which may play an important role in the pathogenesis and maintenance of splanchnic hyperdynamics.
本研究的目的是评估人类肝硬化患者门静脉压力（Pp）升高、门静脉血流量（Qp）以及ETA和ETB受体mRNA表达之间可能存在的相关性。对创伤患者（n = 10）和肝硬化患者（n = 15）的肝组织进行原位杂交和逆转录聚合酶链反应（RT-PCR），以测定ETA和ETB受体mRNA的表达，同时测量这些患者的肝脏血流动力学值。肝硬化患者肝脏样本中这两种转录本的表达明显高于创伤患者肝脏样本中的表达。结果表明，ETA受体mRNA主要位于肝星状细胞（HSCs）以及肝内动脉和门静脉的血管平滑肌细胞中，ETB受体mRNA则位于HSCs、窦状内皮细胞和库普弗细胞中。在肝硬化患者中，ETA和ETB受体mRNA与Pp和Qp之间存在高度显著的直接关系。这表明在人类肝硬化中，肝脏旁分泌内皮素系统可能过度激活，同时伴有ETA和ETB受体mRNA表达增加，这可能在内脏高动力循环的发病机制和维持中起重要作用。
