丹参注射液对实验性兔脊髓损伤的保护作用

[Protective effect of dansheng injection on experimental rabbits' spinal cord injury].

作者信息

Ni Jiang-dong, Ding Ren-kui, Lü Guo-hua

机构信息

Department of Orthopedic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.

出版信息

Hunan Yi Ke Da Xue Xue Bao. 2002 Dec 28;27(6):507-8.

PMID:12658923

Abstract

OBJECTIVE

To investigate the protective effect of Dansheng injection on experimental rabbits' in spinal cord injury.

METHODS

Thirty-two New Zealand rabbits were impacted between T12-T1 with a force of 150 g.cm-1 (10 g x 15 cm). The rabbits were randomly divided into the injury group, monitor group, L-NAME group and Dansheng group. The blood was obtained at 0 h, 4 h, 6 h to separate the serum at the end the injured spinal cord. Cord tissues were obtained for histological examination. Methyl-di-aldehyde (MDA), superoxide dismutase (SOD) and nitro oxide (NO) from the serum and spinal tissues homogenated were tested.

RESULTS

MDA and NO increased in the injury group compared with the others, but SOD significantly decreased in the injury group compared with the others. Histological examination showed that neurons were degenerated and necrosed. The injury group displayed serious changes while the Dansheng group less changes. iNOS expressed in all the groups.

CONCLUSION

Dansheng injection has some protective effect on spinal cord injury in the early stage.

摘要

目的

探讨丹参注射液对实验性兔脊髓损伤的保护作用。

方法

采用150 g.cm-1（10 g×15 cm）的力撞击32只新西兰兔的T12-T1节段。将兔随机分为损伤组、监测组、L-NAME组和丹参组。在0 h、4 h、6 h取血，在损伤脊髓结束时分离血清。取脊髓组织进行组织学检查。检测血清和脊髓组织匀浆中的甲基二醛（MDA）、超氧化物歧化酶（SOD）和一氧化氮（NO）。