[髓复康对脊髓半横断损伤后自由基变化的调节作用研究]

Zhao Yong-qing, Tian Wei, Han Feng-yue

Feng-yue Institute of Orthopaedics & Traumatology, China Academy of TCM, Beijing (100700).

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2004 Nov;24(11):996-8.

OBJECTIVE

To explore the regulatory effect of Suifukang (SFK), a compound Chinese patent drug on the change of free radical in tissue of spinal cord and blood caused by experimental spinal injury.

METHODS

Seventy male adult SD rats were randomly divided into 4 groups, the SFK group (n = 20), the hormone group (n = 20), the control group (n = 20) and the normal group (n = 10). Rats in the former three groups were made into the right hemitransected spinal cord (T12) injury. SFK were given to the rats in the SFK group 3 days before and 2 hrs after modeling via gastrogavage, saline was given to rats in the control group at the same time. As for the rats in the hormone group, methyl-prednisolone 30 mg/kg was given once immediately by peritoneal injection. The rats were sacrificed in two batches (10 of each group in each batch) at 8 hrs and 24 hrs after modeling to obtain the serum and injured spinal cord tissue for determining superoxide dismutase (SOD) activity and malondialdehyde (MDA) content.

RESULTS

(1) As compared with the normal group, SOD activity in serum and spinal tissue of the control group was lower significantly both at 8 hrs and 24 hrs after modeling, but the changes of SOD activity in the SFK and the hormone group were insignificant; (2) Content of MDA in serum and spinal tissue of rats elevated after modeling, which in the control group at 8 hrs after modeling were higher than that in the normal group, also higher than that in the SFK group and the hormone group; but at 24 hrs after modeling, it lowered significantly in both treated groups. The changes of MDA content in serum were similar to those in the spinal tissue.

CONCLUSION

SFK could effectively eliminate the excessive free radical in serum and injured spinal tissue, and raise the capability of antioxidation of organism.

目的

探讨复方中成药髓复康（SFK）对实验性脊髓损伤所致脊髓组织及血液中自由基变化的调节作用。

方法

将70只成年雄性SD大鼠随机分为4组，即SFK组（n = 20）、激素组（n = 20）、对照组（n = 20）和正常组（n = 10）。前三组大鼠制作右侧半横断脊髓（T12）损伤模型。SFK组大鼠于造模前3天及造模后2小时经胃管给药，对照组大鼠同时给予生理盐水。激素组大鼠立即腹腔注射甲泼尼龙30 mg/kg。造模后8小时和24小时分两批处死大鼠（每组每批10只），取血清及损伤脊髓组织测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。

结果

（1）与正常组比较，对照组造模后8小时和24小时血清及脊髓组织中SOD活性均显著降低，但SFK组和激素组SOD活性变化不明显；（2）造模后大鼠血清及脊髓组织中MDA含量升高，对照组造模后8小时高于正常组，也高于SFK组和激素组；但造模后24小时，两个治疗组MDA含量均显著降低。血清中MDA含量变化与脊髓组织相似。