Kwak-Kim J Y H, Chung-Bang H S, Ng S C, Ntrivalas E I, Mangubat C P, Beaman K D, Beer A E, Gilman-Sachs A
Reproductive Medicine, Department of Obstetrics and Gynecology, Finch University of Health Sciences/The Chicago Medical School, 3333 Green Bay Road, N Chicago, IL 60064, USA.
Hum Reprod. 2003 Apr;18(4):767-73. doi: 10.1093/humrep/deg156.
We aimed to study T-helper 1 (Th1) and Th2 intracellular cytokine expression in peripheral blood lymphocytes of women with recurrent spontaneous abortions (RSA) or infertility with multiple implantation failures after IVF cycles.
Twenty-six women with three or more RSA and 23 with two or more IVF failures (14 with no history of spontaneous abortion (SAB) and nine with more than one SAB) comprised the two study groups. Twenty-one non-pregnant healthy multiparous women served as controls. Proportions (%) of lymphocytes containing IFN-gamma, TNF-alpha, IL-4 and IL-10 and the Th1/Th2 ratios of IFN-gamma/IL-4, IFN-gamma/IL-10, TNF-alpha/IL-4 and TNF-alpha/IL-10 in CD3+, CD3+/CD8- (T helper) and CD3+/CD8+ (T suppressor) cells were measured by 4-colour flow cytometry.
RSA women demonstrated significantly higher Th1/Th2 ratios of IFN-gamma/IL-4 (P < 0.01), TNF-alpha/IL-4 and TNF-alpha/IL-10 (P < 0.05 each) in CD3+/CD8- T helper cells than those of controls. The proportion of TNF-alpha producing CD3+/CD8- cells (P < 0.05), and the Th1/Th2 ratios of TNF-alpha/IL-4 (P < 0.05) and TNF-alpha/IL-10 (P < 0.005) in CD3+/CD8- cells were significantly higher in women with multiple IVF failures without SAB as compared with those of controls.
The prevalence of dominant Th1 immune responses in peripheral blood lymphocytes may reflect the systemic contribution of Th1 cytokines to RSA or multiple implantation failures in IVF cycles.
我们旨在研究复发性自然流产（RSA）或体外受精（IVF）周期后多次着床失败的不孕女性外周血淋巴细胞中辅助性T细胞1（Th1）和辅助性T细胞2（Th2）细胞内细胞因子的表达。
26名有三次或更多次RSA的女性和23名有两次或更多次IVF失败的女性（14名无自然流产（SAB）史，9名有不止一次SAB史）组成两个研究组。21名未怀孕的健康经产妇作为对照。通过四色流式细胞术测量CD3 +、CD3 + / CD8 -（辅助性T细胞）和CD3 + / CD8 +（抑制性T细胞）细胞中含干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-4（IL-4）和白细胞介素-10的淋巴细胞比例（%）以及IFN-γ/ IL-4、IFN-γ/ IL-10、TNF-α/ IL-4和TNF-α/ IL-10的Th1 / Th2比值。
RSA女性CD3 + / CD8 -辅助性T细胞中IFN-γ/ IL-4（P < 0.01）、TNF-α/ IL-4和TNF-α/ IL-10（各P < 0.05）的Th1 / Th2比值显著高于对照组。与对照组相比，无SAB的多次IVF失败女性中产生TNF-α的CD3 + / CD8 -细胞比例（P < 0.05）以及CD3 + / CD8 -细胞中TNF-α/ IL-4（P < 0.05）和TNF-α/ IL-10（P < 0.005）的Th1 / Th2比值显著更高。
外周血淋巴细胞中占主导的Th1免疫反应的普遍存在可能反映了Th1细胞因子对RSA或IVF周期中多次着床失败的全身性影响。
