技术说明：从奶牛瘤胃样本提取的DNA中原生动物18S rDNA序列的特异性PCR扩增
Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows.
作者信息
Karnati S K R, Yu Z, Sylvester J T, Dehority B A, Morrison M, Firkins J L
机构信息
Department of Animal Sciences, The Ohio State University, Columbus 43210, USA.
出版信息
J Anim Sci. 2003 Mar;81(3):812-5. doi: 10.2527/2003.813812x.
A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.
设计了一种原生动物特异性引物（P-SSU-342f），并将其与真核生物特异性引物配对，以从喂食混合草料：谷物日粮或苜蓿干草的奶牛瘤胃液中扩增编码原生动物小亚基（SSU）核糖体RNA的1360 bp DNA片段。克隆测序表明，P-SSU-342f对瘤胃原生动物具有特异性，稍加修改后，该引物将有助于瘤胃原生动物的生态学研究。
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