Shin E C, Cho K M, Lim W J, Hong S Y, An C L, Kim E J, Kim Y K, Choi B R, An J M, Kang J M, Kim H, Yun H D
Division of Applied Life Science, Gyeongsang National University, Chinju, South Korea.
J Appl Microbiol. 2004;97(2):378-83. doi: 10.1111/j.1365-2672.2004.02304.x.
To examine the diversity of protozoa in the rumen contents of cow.
Protozoa that inhabit the rumen were detected by PCR using protozoan-specific primers. Libraries of protozoan rDNA sequences were constructed from rumen fluid, solid tissues and epithelium. Twenty-three clones isolated from rumen fluid fell into two genera identified as Entodinium (69.6% of clones) and Epidinium (31.4% of clones). Of the clones isolated from rumen fluid, a moderate number were unidentifiable (30.4%).
The predominant protozoan genus identified in the whole rumen belonged to the Entodinium group (81.1%). Protozoa were not detected in the rumen epithelium.
These findings suggest that rumen fluid and solid tissues contain different protozoan populations that may play specific roles in rumen function. Quantitative PCR techniques and a more specific set of phylogenetic probes that distinguish between protozoan species are needed to determine the significance of newly identified groups and to determine the distribution of identified protozoan clusters in rumen microbial communities.
研究奶牛瘤胃内容物中原生动物的多样性。
使用原生动物特异性引物通过聚合酶链反应（PCR）检测瘤胃中的原生动物。从瘤胃液、固体组织和上皮构建原生动物核糖体DNA（rDNA）序列文库。从瘤胃液中分离出的23个克隆分为两个属，分别鉴定为内毛虫属（占克隆的69.6%）和艾美球虫属（占克隆的31.4%）。从瘤胃液中分离出的克隆中，有相当数量无法鉴定（30.4%）。
在整个瘤胃中鉴定出的主要原生动物属属于内毛虫属组（81.1%）。在瘤胃上皮中未检测到原生动物。
这些发现表明瘤胃液和固体组织含有不同的原生动物种群，它们可能在瘤胃功能中发挥特定作用。需要定量PCR技术和一套更特异的系统发育探针来区分原生动物物种，以确定新鉴定群体的意义，并确定已鉴定原生动物簇在瘤胃微生物群落中的分布。
