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一种用放射性硒对肝片吸虫毛蚴进行体内标记的技术。

A technique for in vivo labelling of Fasciola hepatica miracidia with radioselenium.

作者信息

Nansen P, Christensen N O, Frandsen F

出版信息

Z Parasitenkd. 1976 Mar 31;49(1):73-80. doi: 10.1007/BF00445019.

DOI:10.1007/BF00445019
PMID:1266290
Abstract

Radioactivity is incorporated in Fasciola hepatica and its eggs when 75Semethionine is administered intraperitoneally in chronically infected mice. Maximum egg-bound radioactivity is achieved 2-3 days after the administration of the labelled aminoacid. Seventy-three per cent of the radioactivity of the egg is recovered in the free-swimming miracidium. There appears to be no altered motility or infectivity to snails of miracidia made radioactive at a level corresponding to 6 pCi per larva. Suggestions are made for the application of labelled larvae in studies on various aspects of the parasite-snail contact.

摘要

当向慢性感染的小鼠腹腔注射75硒蛋氨酸时,放射性物质会掺入肝片吸虫及其虫卵中。在给予标记氨基酸后2 - 3天,虫卵结合的放射性达到最大值。虫卵中73%的放射性在自由游动的毛蚴中被回收。对于每只幼虫放射性水平相当于6皮居里的放射性毛蚴,其对蜗牛的运动性或感染性似乎没有改变。文中针对标记幼虫在寄生虫与蜗牛接触各方面研究中的应用提出了建议。

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本文引用的文献

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UTILIZATION OF METHIONINE BY THE LIVER FLUKE, FASCIOLA HEPATICA.肝片吸虫(Fasciola hepatica)对蛋氨酸的利用
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ON THE ULTRASTRUCTURE OF THE OVARY OF THE LIVER FLUKE (FASCIOLA HEPATICA L.).
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Host-location by trematode miracidia.吸虫毛蚴对宿主的定位
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[Cytological and cytochemical study of cells of the germinative line in Fasciola hepatica].[肝片吸虫生发系细胞的细胞学与细胞化学研究]
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Acta Vet Scand. 1974;15(1):144-6. doi: 10.1186/BF03547503.