Genc Sermin, Kizildag Sefa, Genc Kursad, Ates Halil, Atabey Nese
Department of Medical Biology and Medical Genetics, School of Medicine, Dokuz Eylul University, Inciralti, 35340, Izmir, Turkey.
Immunol Lett. 2003 Feb 3;85(3):271-4. doi: 10.1016/s0165-2478(02)00245-6.
In this study, it is reported that neonatal murine microglia and N9 murine microglial cell line express tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). TRAIL protein and mRNA expression in murine microglia greatly upregulate upon stimulation with interferon gamma (IFNgamma) or lipopolysaccharide (LPS) as revealed by immunoprecipitation-immunoblotting, reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry techniques. IFNgamma and LPS act synergistically to induce TRAIL expression on both translational and transcriptional levels. The upregulated microglial TRAIL in inflammatory conditions may involve in the cytotoxic effect of these cells and play a role in neurodegenerative processes.
在本研究中，据报道新生鼠小胶质细胞和N9鼠小胶质细胞系表达肿瘤坏死因子（TNF）相关凋亡诱导配体（TRAIL）。免疫沉淀-免疫印迹、逆转录聚合酶链反应（RT-PCR）和流式细胞术技术显示，用γ干扰素（IFNγ）或脂多糖（LPS）刺激后，鼠小胶质细胞中TRAIL蛋白和mRNA表达大幅上调。IFNγ和LPS协同作用，在翻译和转录水平上诱导TRAIL表达。炎症条件下上调的小胶质细胞TRAIL可能参与这些细胞的细胞毒性作用，并在神经退行性过程中发挥作用。
