Mackenzie I C
Am J Anat. 1975 Oct;144(2):127-36. doi: 10.1002/aja.1001440202.
Epidermal Langerhans cells may be identified by light microscopy by their strongly positive reaction following incubation for ATPase activity. Intact sheets of epidermis from mice killed at various time intervals following a single pulse label of H3-thymidine were incubated to demonstrate ATPase activity and subsequently processed for autoradiography. In specimens taken one hour after labelling, many basal keratinocytes were labelled but very few ATPase-positive dendritic cells. At subsequent time periods a few pairs of labelled ATPase-positive cells were found but individually labelled cells were not observed. The findings suggest that epidermal Langerhans cells form a very stable (labelling index less than 0.01%) self-replicating population which divides to maintain cell spacing during growth. No evidence was found for migration and interchange of Langerhans cells with the connective tissue, or for an origin of Langerhans cells by transformation of another cell type.
通过光学显微镜观察，在孵育以检测ATP酶活性后，表皮朗格汉斯细胞会呈现强烈的阳性反应，从而得以识别。对在单次脉冲标记H3-胸苷后的不同时间间隔处死的小鼠所取的完整表皮片进行孵育，以显示ATP酶活性，随后进行放射自显影处理。在标记后一小时所取的标本中，许多基底角质形成细胞被标记，但ATP酶阳性的树突状细胞极少。在随后的时间段里，发现了几对标记的ATP酶阳性细胞，但未观察到单个标记的细胞。这些发现表明，表皮朗格汉斯细胞形成了一个非常稳定的（标记指数小于0.01%）自我复制群体，在生长过程中进行分裂以维持细胞间距。未发现朗格汉斯细胞与结缔组织之间有迁移和交换的证据，也未发现朗格汉斯细胞起源于另一种细胞类型转化的证据。
