Gaunt Stephen D., Prescott-Mathews Judith S., King William W., Scholl Daniel T.
Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.
Vet Clin Pathol. 1995;24(2):64-67. doi: 10.1111/j.1939-165x.1995.tb00938.x.
The clinical hematology practices utilized at veterinary teaching hospitals and private veterinary diagnostic laboratories were surveyed using a questionnaire. The hematology caseload at private diagnostic laboratories was larger, and comprised predominantly of canine and feline submissions. The Coulter S Plus IV and Serono Baker 9000 were the hematology analyzers used most frequently at veterinary medical laboratories. The Abbott Cell-Dyn 3500, a multispecies analyzer capable of leukocyte differential counting, was utilized more by private laboratories. Commercial hematology control reagents were used at all laboratories; teaching hospital laboratories more often used reagents supplied by the manufacturer of the analyzer. A greater percentage of private diagnostic laboratories participated in the external quality assurance programs offered by Veterinary Laboratory Association and College of American Pathologists. While private diagnostic laboratories retained the EDTA blood specimens longer after initial testing, the teaching hospital laboratories retained blood smears and complete blood count reports longer. The complete blood count reports at veterinary teaching laboratories more often included red blood cell volume distribution width, mean platelet volume, manual hematocrit, plasma protein, and leukocyte differentials as absolute concentrations. The laboratory practices utilized by these veterinary medical laboratories were generally similar, and differences were attributed to divergent emphasis on economic accountability and clinical investigation.
通过问卷调查的方式,对兽医教学医院和私立兽医诊断实验室所采用的临床血液学实践进行了调查。私立诊断实验室的血液学病例量更大,主要包括犬类和猫类样本。库尔特S Plus IV和赛诺菲德贝克9000是兽医医学实验室最常使用的血液分析仪。私立实验室更多地使用了雅培Cell-Dyn 3500,这是一种能够进行白细胞分类计数的多物种分析仪。所有实验室都使用商业血液学控制试剂;教学医院实验室更常使用分析仪制造商提供的试剂。更高比例的私立诊断实验室参与了由兽医实验室协会和美国病理学家学会提供的外部质量保证计划。虽然私立诊断实验室在初次检测后保留乙二胺四乙酸(EDTA)血液标本的时间更长,但教学医院实验室保留血涂片和全血细胞计数报告的时间更长。兽医教学实验室的全血细胞计数报告更常包括红细胞体积分布宽度、平均血小板体积、手工血细胞比容、血浆蛋白以及作为绝对浓度的白细胞分类计数。这些兽医医学实验室所采用的实验室实践总体上相似,差异归因于对经济责任和临床研究的不同侧重点。