Babich T V, Kovalenko E O, Nahorna S S, Het'man K I, Pidhors'kyĭ V S
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiol Z. 2002 Nov-Dec;64(6):41-6.
Hemagglutinating properties of the culture liquid and cells of 451 strains, 24 species, 7 genera of the museum and fresh yeast cultures have been investigated. The researchers have distinguished 333 strains (74%) capable for production of extracellular lectins and those bound to the cell surface with activity about 512-1024 hemagglutinating units. The extracellular lectins were found in 41% of lectin-synthesizing strains; the highest amount of their producers were found among representatives of genera Saccharomyces, Kluyveromyces, Cryptococcus. Strains of Debaryomyces genus mainly synthesized lectins of the bound form, and the yeast of Pichia genus--lectins of the both types. Affinity of the extracellular lectin Saccharomyces cerevisiae UCM Y-530 for D-gluconic acid has been established.
对博物馆保存的451株、24种、7属菌株及新鲜酵母培养物的培养液和细胞的血凝特性进行了研究。研究人员区分出333株(74%)能够产生细胞外凝集素的菌株,这些凝集素与细胞表面结合,活性约为512 - 1024个血凝单位。在41%的凝集素合成菌株中发现了细胞外凝集素;其产生者数量最多的是在酿酒酵母属、克鲁维酵母属、隐球菌属的代表菌株中。德巴利酵母属的菌株主要合成结合型凝集素,毕赤酵母属的酵母则合成两种类型的凝集素。已确定酿酒酵母UCM Y - 530的细胞外凝集素对D - 葡萄糖酸具有亲和力。