Ermakova I T, Lozinov A B
Mikrobiologiia. 1976;45(4):640-5.
Yeast cultures, capable and incapable fo growth on hydrocarbons, were tested for their ability to oxidize aliphatic alcohols and acids, intermediate products of n-alkane degradation. The cultures belonging to 7 genera and 13 species did not grow on liquid paraffins and tetradecane. However, they grew on tetradecanol and oxidized aliphatic alcohols and fatty acids, the pattern of oxidation of the substrates being the same as in the case of alkane-oxidizing yeast cultures grown on glucose. None of 45 yeast strains (12 genera and 36 species) which did not grow on paraffin was able to oxidize tetradecane. As follows from this and earlier evidence (Lozinov et al., 1976a), the only reaction typical of yeasts capable of growth on n-alkanes is the oxigenase reaction of their oxidation to alcohols.
对能够和不能够在烃类上生长的酵母培养物进行了测试,以检测它们氧化脂肪醇和酸(正构烷烃降解的中间产物)的能力。属于7个属和13个种的培养物不能在液体石蜡和十四烷上生长。然而,它们能在十四烷醇上生长并氧化脂肪醇和脂肪酸,底物的氧化模式与在葡萄糖上生长的烷烃氧化酵母培养物的情况相同。45株不能在石蜡上生长的酵母菌株(12个属和36个种)中,没有一株能够氧化十四烷。由此以及早期证据(洛齐诺夫等人,1976年a)可知,能够在正构烷烃上生长的酵母的唯一典型反应是将其氧化为醇的加氧酶反应。