Liu Jing-Han, Gao Dayong, He Li-Qun, Moey Lip Kean, Hua Kegang, Liu Zuo-Bin
Department of Blood Transfusion, General Hospital of PLA, Centre for Clinic Blood Transfusion Medicine of PLA, Beijing, 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2003 Feb;11(1):92-5.
In order to acquire freezing model of the cryopretective solution (NaCl-propylene glycol-water ternary system) for platelet, the melting points (T(f)) of this cryopretective solutions with different solute concentration and different ratio of PG mass to NaCl mass were measured by using a differential scanning calorimeter (DSC), and these experimental data were fitting by computer. An empirical equation was derived which characterized the Tf as a function of the solute concentration and the ratio of PG mass to NaCl mass inside this solution. It was concluded that the equilibrium freezing model for human platelets in this system could be used to instruct platelet cryopreserving techniques.
为获得血小板低温保护剂（NaCl-丙二醇-水三元体系）的冻结模型，采用差示扫描量热仪（DSC）测定了不同溶质浓度及不同PG与NaCl质量比的该低温保护剂溶液的熔点（T(f)），并对这些实验数据进行计算机拟合。推导出一个经验方程，该方程将Tf表征为该溶液中溶质浓度和PG与NaCl质量比的函数。得出该体系中人体血小板的平衡冻结模型可用于指导血小板冷冻保存技术的结论。
