Rothschild H, Germershausen J, Suskind S R
Biochem Genet. 1975 Jun;13(5-6):283-300. doi: 10.1007/BF00485814.
Genetic analysis of a number of cycloheximide-resistant mutants of Neurospora crassa has shown that resistance is controlled by several genes. Two of these appear to be located on linkage group V. Resistance to the antibiotic is dominant in wild-type-mutant heterokaryons. Two types of cycloheximide-resistant mutants were isolated: one type exhibited colonial morphology only when grown in the presence of cycloheximide and the other type maintained normal morphology even at high concentrations of the antibiotic. Reconstitution experiments with supernatant solutions and 80S monosomes prepared from wild-type and resistant mutant strains indicated that the property of cycloheximide resistance most likely is associated with the ribosomes. No electrophoretic or serological differences were found between the ribosomal proteins of the wild-type and resistant mutants.
对粗糙脉孢菌的多个抗放线菌酮突变体进行的遗传分析表明，抗性由多个基因控制。其中两个基因似乎位于连锁群V上。在野生型 - 突变体异核体中，对抗生素的抗性是显性的。分离出了两种类型的抗放线菌酮突变体：一种类型仅在放线菌酮存在下生长时表现出菌落形态，另一种类型即使在高浓度抗生素下也保持正常形态。用从野生型和抗性突变体菌株制备的上清液和80S单体进行的重组实验表明，抗放线菌酮特性很可能与核糖体有关。在野生型和抗性突变体的核糖体蛋白之间未发现电泳或血清学差异。
