Martegani E, Popolo L, Alberghina L, Sturani E
Biochim Biophys Acta. 1980 Dec 11;610(2):318-30. doi: 10.1016/0005-2787(80)90013-1.
The addition of cycloheximide (0.02 micrograms/ml) to exponentially growing cultures of Neurospora crassa causes a reduction in growth rate and a decrease in the rate of protein accumulation, due to a partial inhibition of protein synthesis, while RNA accumulation is unaffected for about 1 h. Thus, an increased RNA:protein ratio is established in the presence of the inhibitor. RNA that accumulates during treatment with cycloheximide has the same characteristics as that of the control cultures and this, together with the enhancement of the relative rate of synthesis of ribosomal proteins induced by cycloheximide, seems to indicate that more mature ribosomes are present in cycloheximide-treated cultures. The endocellular level of several amino acids begins to increase significantly only 60 min after cycloheximide addition. A possible explanation of the stimulation of ribosome production induced by cycloheximide is given in terms of the existence of a feed-back mechanism controlling ribosome synthesis.
向指数生长的粗糙脉孢菌培养物中添加放线菌酮（0.02微克/毫升）会导致生长速率降低以及蛋白质积累速率下降，这是由于蛋白质合成受到部分抑制，而RNA积累在约1小时内不受影响。因此，在抑制剂存在的情况下会建立起增加的RNA：蛋白质比率。在用放线菌酮处理期间积累的RNA与对照培养物的RNA具有相同的特征，这一点，再加上放线菌酮诱导的核糖体蛋白质合成相对速率的提高，似乎表明在用放线菌酮处理的培养物中存在更多成熟的核糖体。仅在添加放线菌酮60分钟后，几种氨基酸的细胞内水平才开始显著增加。根据控制核糖体合成的反馈机制的存在，给出了对放线菌酮诱导核糖体产生刺激的一种可能解释。
