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The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.乙炔-乙烯法测定固氮作用中的 N2 固定：实验室和现场评价。
Plant Physiol. 1968 Aug;43(8):1185-207. doi: 10.1104/pp.43.8.1185.
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Rhizobium leguminosarum bv. trifolii PssP protein is required for exopolysaccharide biosynthesis and polymerization.三叶草根瘤菌的PssP蛋白是胞外多糖生物合成和聚合所必需的。
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Isolation and sequencing of Rhizobium leguminosarum Bv. Trifolii PssN, PssO and PssP genes encoding the proteins involved in polymerization and translocation of exopolysaccharide.豆科根瘤菌三叶草根瘤菌生物变种中参与胞外多糖聚合和转运的蛋白质编码基因PssN、PssO和PssP的分离与测序
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The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific tyrosine phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein.苜蓿中华根瘤菌产生的琥珀聚糖的分子量分布受ExoP蛋白胞质结构域的特定酪氨酸磷酸化和ATP酶活性的影响。
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Membrane topology and insertion of membrane proteins: search for topogenic signals.膜蛋白的膜拓扑结构与插入：寻找拓扑信号
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Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions.基于卷曲螺旋区域的存在对Wzz/MPA1/MPA2蛋白进行评估。
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Structural characterization of the symbiotically important low-molecular-weight succinoglycan of Sinorhizobium meliloti.苜蓿中华根瘤菌共生重要的低分子量琥珀酰聚糖的结构表征
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VHMPT: a graphical viewer and editor for helical membrane protein topologies.
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[Structure-functional organization of exopolysaccharide biosynthetic genes in Rhizobium leguminosarum bv. viciae VF39].[豌豆根瘤菌蚕豆生物变种VF39中胞外多糖生物合成基因的结构-功能组织]
Mol Biol (Mosk). 1998 Sep-Oct;32(5):797-804.

豌豆根瘤菌三叶草生物型TA1菌株中I型胞外多糖转运系统的跨膜蛋白组分PssT的膜拓扑结构。

Membrane topology of PssT, the transmembrane protein component of the type I exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii strain TA1.

作者信息

Mazur Andrzej, Król Jarosław E, Marczak Małgorzata, Skorupska Anna

机构信息

Department of General Microbiology, M. Curie-Skłodowska University, 20-033 Lublin, Poland.

出版信息

J Bacteriol. 2003 Apr;185(8):2503-11. doi: 10.1128/JB.185.8.2503-2511.2003.

DOI:10.1128/JB.185.8.2503-2511.2003
PMID:12670974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC152602/
Abstract

The pssT gene was identified as the fourth gene located upstream of the pssNOP gene cluster possibly involved in the biosynthesis, polymerization, and transport of exopolysaccharide (EPS) in Rhizobium leguminosarum bv. trifolii strain TA1. The hydropathy profile and homology searches indicated that PssT belongs to the polysaccharide-specific transport family of proteins, a component of the type I system of the polysaccharide transport. The predicted membrane topology of the PssT protein was examined with a series of PssT-PhoA fusion proteins and a complementary set of PssT-LacZ fusions. The results generally support a predicted topological model for PssT consisting of 12 transmembrane segments, with amino and carboxyl termini located in the cytoplasm. A mutant lacking the C-terminal part of PssT produced increased amounts of total EPS with an altered distribution of high- and low-molecular-weight forms in comparison to the wild-type RtTA1 strain. The PssT mutant produced an increased number of nitrogen fixing nodules on clover.

摘要

pssT基因被鉴定为位于豆科根瘤菌三叶草生物型TA1菌株中可能参与胞外多糖（EPS）生物合成、聚合和转运的pssNOP基因簇上游的第四个基因。亲水性图谱和同源性搜索表明，PssT属于多糖特异性转运蛋白家族，是多糖转运I型系统的一个组成部分。使用一系列PssT-PhoA融合蛋白和一组互补的PssT-LacZ融合蛋白检测了PssT蛋白的预测膜拓扑结构。结果总体上支持了PssT的预测拓扑模型，该模型由12个跨膜区段组成，氨基和羧基末端位于细胞质中。与野生型RtTA1菌株相比，缺失PssT C末端部分的突变体产生的总EPS量增加，高分子量和低分子量形式的分布发生改变。PssT突变体在三叶草上产生的固氮根瘤数量增加。