Thornton Catherine A, Capristo Carlo C, Power Lynsey L, Holloway Judith A, Popplewell Eleanor J, Diaper Norma D, Warner John O
Infection, Inflammation and Repair, School of Medicine, University of Southampton, UK.
Pediatr Res. 2003 Jul;54(1):120-4. doi: 10.1203/01.PDR.0000069704.25043.BA. Epub 2003 Apr 2.
With increasing interest in the role of fetal programming in child and adulthood diseases, and therefore interest in the measurement of various factors at birth, it is essential to ascertain whether the factors of interest show any gestation- or parturition-associated changes. We have investigated whether mode of delivery influenced T-cell phenotype and function (CD4+) as has been described for monocytes and neutrophils. Interferon-gamma production in response to either the mitogen phytohemagglutinin or anti-CD2/CD3/CD28 F(ab')3 was significantly reduced by neonatal mononuclear cells compared with adult cells but did not differ with mode of delivery at term (normal vaginal delivery versus elective lower-segment cesarean section). Likewise, anti-CD2/CD3/CD28-stimulated IL-2 production by the neonate was lower than adult levels but did not differ with mode of delivery. The expression of common T-cell activation markers (CD25, MHC class II, CD69, CD62L, CD11a, CD44, and CD49d) was examined. Only CD62L (L-selectin) expression was significantly different, with fewer adult T cells expressing this surface antigen compared with neonatal T cells (p < 0.0003), and significantly more T cells from lower-segment cesarean section than normal vaginal delivery were positive for CD62L (p = 0.012). sCD62L levels were significantly lower in cord plasma compared with adult plasma but did not differ with mode of delivery. Thus the phenotype and function of cord blood T cells did not differ greatly with mode of delivery, but possible differences for the marker of interest should always be assessed. Furthermore, although there was no significant difference with mode of delivery for all markers, except CD62L, the variation in the normal vaginal delivery samples, as for the adults, was greater than in the lower-segment cesarean section samples, indicating that the effects of length of labor and stress at delivery may well be relevant.
随着人们对胎儿编程在儿童和成人疾病中作用的兴趣日益增加，因此对出生时各种因素的测量也产生了兴趣，确定感兴趣的因素是否显示出任何与妊娠或分娩相关的变化至关重要。我们研究了分娩方式是否会影响T细胞表型和功能（CD4 +），单核细胞和中性粒细胞已有相关描述。与成年细胞相比，新生儿单核细胞对丝裂原植物血凝素或抗CD2 / CD3 / CD28 F(ab')3的干扰素-γ产生明显降低，但足月时的分娩方式（正常阴道分娩与选择性下段剖宫产）并无差异。同样，新生儿抗CD2 / CD3 / CD28刺激的IL-2产生低于成人水平，但与分娩方式无关。检测了常见T细胞活化标志物（CD25、MHC II类、CD69、CD62L、CD11a、CD44和CD49d）的表达。只有CD62L（L-选择素）表达存在显著差异，与新生儿T细胞相比，表达这种表面抗原的成年T细胞较少（p < 0.0003），并且下段剖宫产的T细胞中CD62L阳性的比正常阴道分娩的明显更多（p = 0.012）。脐血血浆中的sCD62L水平与成人血浆相比显著较低，但与分娩方式无关。因此，脐血T细胞的表型和功能在分娩方式上差异不大，但应始终评估感兴趣标志物的可能差异。此外，尽管除CD62L外所有标志物在分娩方式上均无显著差异，但正常阴道分娩样本中的变异，与成人样本一样，大于下段剖宫产样本，这表明分娩时长和分娩时应激的影响可能很重要。
