Kumagai Mitsutake, Sato Iwao
Department of Anatomy, School of Dentistry at Tokyo, Nippon Dental University, Tokyo, Japan.
Cells Tissues Organs. 2003;173(3):184-90. doi: 10.1159/000069472.
We examined the distribution of fibronectin and collagen types I and III in human fetuses under a confocal laser scanning microscope using immunohistochemical staining (at 16, 20, 24, 28, 32 weeks' gestation). The collagen types I and III form the collagenous matrix components in the connective tissue of the parotid and submandibular glands. These extracellular matrix components were detected at various stages around the terminal portion and in the capsule-like connective tissue of the parotid and submandibular glands. However, the extracellular matrix components in the connective tissue around the terminal portion had a stronger reaction than those in the capsule-like connective tissue found on the fringe of the terminal portion. The collagen type I of the parotid gland at 16 weeks' gestation had a weaker reaction than that of the submandibular gland. When results of the reaction at other stages and other extracellular matrix components of the two salivary glands were compared, collagen type I appeared as early as at 16 weeks' gestation in either gland. Since collagen type I serves as the core connective tissue, these observations suggest that the formation of connective tissue around the parotid gland occurs before or after 16 weeks' gestation lagging behind that of the submandibular gland.
我们使用免疫组织化学染色法（在妊娠16、20、24、28、32周时），在共聚焦激光扫描显微镜下检查了人胎儿中纤连蛋白以及I型和III型胶原蛋白的分布。I型和III型胶原蛋白构成腮腺和颌下腺结缔组织中的胶原基质成分。这些细胞外基质成分在腮腺和颌下腺终末部周围的各个阶段以及囊状结缔组织中均有检测到。然而，终末部周围结缔组织中的细胞外基质成分比终末部边缘处的囊状结缔组织中的反应更强。妊娠16周时腮腺的I型胶原蛋白反应比颌下腺弱。当比较两个唾液腺在其他阶段的反应结果以及其他细胞外基质成分时，I型胶原蛋白在两个腺体中最早在妊娠16周时出现。由于I型胶原蛋白是结缔组织的核心成分，这些观察结果表明，腮腺周围结缔组织的形成发生在妊娠16周之前或之后，落后于颌下腺。
