Liu Gang, Lu Guangxiu, Fu Junjiang, Liu Shangfeng, Xing Xiaowei, Li Luyun
Human Reproductive Engineering Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Apr;20(2):107-10.
To clone a novel gene which is related to human testis spermatogenesis apoptosis.
To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.
The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.
A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
克隆一个与人类睾丸生精细胞凋亡相关的新基因。
为了从人睾丸cDNA文库中快速获得人类新基因全长cDNA序列，设计基因特异性引物和载体特异性引物用于巢式聚合酶链反应。进行测序并分析结果。
作者利用一个cDNA片段（GenBank登录号BE644537）作为电子探针，从人睾丸cDNA文库中发现了TSARG3基因（GenBank登录号AF419291），该基因在隐睾症中发生了显著变化，代表一个新基因。此外，用同样的方法鉴定出了该基因的小鼠同源物（GenBank登录号AF419292）。
克隆了一个名为TSARG3的新基因。认为该新基因的功能与人类睾丸生精细胞凋亡有关。
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