Fu Jun-Jiang, Lu Guang-Xiu, Li Lu-Yun, Liu Gang, Xing Xiao-Wei, Liu Shang-Feng
Laboratory of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China.
Yi Chuan Xue Bao. 2003 Jan;30(1):25-9.
Spermatogenesis cell apoptosis is a very complex process, which needs many molecules to take part in the programmable death of cells in testis. At present, research of apoptosis for spermatogenesis cell is at the primary step. It is very important to clone spermatogenesis cell apoptosis related genes and spermatogenesis genes in testis. Applying the bioinformatics and experiment technique, we have cloned human and mouse novel gene cDNA sequences--human testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) and mouse testis and spermatogenesis cell apoptosis related gene 1(Mtsarg1) from human and mouse testis cDNA library respectively, using a cDNA fragment (GenBank accession number: BE644538) as an electronic probe, which was significantly changed in expression in cryptorchidism. The GeneBank accession numbers of Mtsarg1 and TSARG1 are AF399971 and AY032925 (NM_139073), respectively. The Mtsarg1 has a 55% identity and 61% similarity with TSARG1 at the amino acid level, which did not share significant homology with any other known protein in databases. The full-length cDNA of TSARG1 gene is 973 bp, including 549 bp open reading frame(ORF) and coding 183 amino acids, whereas the full-length cDNA of Mtsarg1 gene is 1103 bp, including 576 bp ORF and coding 192 amino acids. The predicted molecule weight of TSARG1 is 19948.61 Dolton, and the deduced iso-electric point is 10.24, whereas the Mtsarg1 is 20875.93 and is 9.83, being alkaline proteins. RT-PCR analysis showed that Mtsarg1 was expressed significantly in testis and faintly in epididymis in the ten tissues of testis, ovary, spleen, kidney, lung, heart, brain, epididymis, liver and skeletal muscle in mouse, while it wasn't expressed in the other eight tissues. Therefore, our results suggested that Mtsarg1 and TSARG1 would be pay potential roles in spermatogenesis cell apoptosis or spermatogenesis.
精子发生细胞凋亡是一个非常复杂的过程，需要许多分子参与睾丸中细胞的程序性死亡。目前，对精子发生细胞凋亡的研究尚处于初级阶段。克隆睾丸中精子发生细胞凋亡相关基因和精子发生基因非常重要。应用生物信息学和实验技术，我们分别从人和小鼠睾丸cDNA文库中克隆了人和小鼠的新基因cDNA序列——人睾丸和精子发生细胞凋亡相关基因1（TSARG1）和小鼠睾丸和精子发生细胞凋亡相关基因1（Mtsarg1），使用一个在隐睾症中表达有显著变化的cDNA片段（GenBank登录号：BE644538）作为电子探针。Mtsarg1和TSARG1的GenBank登录号分别为AF399971和AY032925（NM_139073）。Mtsarg1与TSARG1在氨基酸水平上有55%的同一性和61%的相似性，与数据库中任何其他已知蛋白质均无显著同源性。TSARG1基因的全长cDNA为973 bp，包括549 bp的开放阅读框（ORF），编码183个氨基酸，而Mtsarg1基因的全长cDNA为1103 bp，包括576 bp的ORF，编码192个氨基酸。TSARG1的预测分子量为19948.61道尔顿，推导的等电点为10.24，而Mtsarg1为20875.93，等电点为9.83，均为碱性蛋白。RT-PCR分析表明，Mtsarg1在小鼠睾丸、卵巢、脾脏、肾脏、肺、心脏、大脑、附睾、肝脏和骨骼肌这10个组织中，在睾丸中表达显著，在附睾中表达较弱，而在其他8个组织中不表达。因此，我们的结果表明Mtsarg1和TSARG1可能在精子发生细胞凋亡或精子发生中发挥潜在作用。
