Mori Koji, Sparling Richard, Hatsu Masahiro, Takamizawa Kazuhiro
Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Can J Microbiol. 2003 Jan;49(1):28-36. doi: 10.1139/w03-006.
At a sea-based, solid waste disposal site, methanogenic organisms were quantified by molecular approaches. The samples collected for analysis were from anaerobic leachate of the landfill site. When the DNA extracted from the leachate was examined by a quantitative PCR method using domain-specific 16S rDNA primers, archaeal DNA represented 2-3% of the total extracted DNA. On the basis of cloning and sequence comparison of the archaeal PCR products, more than half of the sequences belonged to Euryarchaeota, particularly relatives of the genus Methanosaeta. The cloning analysis suggested that the majority of methane emitted from the landfill site originated from the acetate-utilizing Methanosaeta.
在一个海上固体废弃物处理场，通过分子方法对产甲烷生物进行了定量分析。用于分析的样本取自该垃圾填埋场的厌氧渗滤液。当使用域特异性16S rDNA引物通过定量PCR方法检测从渗滤液中提取的DNA时，古菌DNA占总提取DNA的2 - 3%。基于古菌PCR产物的克隆和序列比较，超过一半的序列属于广古菌门，尤其是甲烷八叠球菌属的亲缘种。克隆分析表明，该垃圾填埋场排放的大部分甲烷源自利用乙酸盐的甲烷八叠球菌。
