Formisano S, Lamas L, Vignal A, Cortese F, Schneider A B
C R Seances Soc Biol Fil. 1975;169(1):60-5.
Iodoaminoacid content (iodothyronines, T3 and T4, and iodotyrosines, MIT and DIT) has been determined in enzymatic hydrolysates of thyroglobulin Tg 19S of different iodine content (0.3-0.9%) isolated from equilibrium labeled rats. Preparative equilibrium centrifugation in RbCl density gradients of pure thyroglobulin was used to obtain protein fractions of largely different iodine content (0.2-1.2% I). Thin layer chromatography of total hydrolysates demonstrated that the distribution of iodoaminoacids depends on the total iodine content of each fraction. It is concluded, in agreement with previous results, that the native structure of Tg is an important factor in the regulation of hormone biosynthesis and that even at low iodination levels of Tg. T3 and T4 are synthesized.
已测定从平衡标记大鼠分离出的不同碘含量（0.3 - 0.9%）的甲状腺球蛋白Tg 19S酶水解产物中的碘氨基酸含量（甲状腺原氨酸、T3和T4，以及碘酪氨酸、MIT和DIT）。采用在RbCl密度梯度中对纯甲状腺球蛋白进行制备性平衡离心的方法，以获得碘含量差异很大（0.2 - 1.2% I）的蛋白质组分。总水解产物的薄层色谱表明，碘氨基酸的分布取决于每个组分的总碘含量。与先前结果一致，得出的结论是，Tg的天然结构是激素生物合成调节中的一个重要因素，并且即使在Tg的低碘化水平下，T3和T4也会合成。
