Suppr超能文献

鸡晶状体发育过程中L-Maf与Sox2在δ-晶状体蛋白基因表达上的协同作用。

Cooperative action between L-Maf and Sox2 on delta-crystallin gene expression during chick lens development.

作者信息

Shimada Naoko, Aya-Murata Tomoko, Reza Hasan Mahmud, Yasuda Kunio

机构信息

Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.

出版信息

Mech Dev. 2003 Apr;120(4):455-65. doi: 10.1016/s0925-4773(03)00002-9.

Abstract

Lens development is regulated by a variety of transcription factors with distinct properties. The lens-specific transcription factor, L-Maf, is essential for lens formation and induces lens-specific markers, such as the crystallin genes. In this study, we analyzed the mechanism by which L-Maf regulates delta-crystallin expression. Misexpression of L-Maf in the head ectoderm of lens placode-forming embryos by in ovo electroporation induced delta-crystallin only in the region surrounding the lens. To define this restricted expression, we misexpressed L-Maf together with other transcription factors implicated in delta-crystallin expression. Sox2 plus L-Maf expanded the delta-crystallin-inducible domain to the entire head ectoderm and simultaneously increased the quantity of delta-crystallin mRNA expressed. In contrast, co-expression of L-Maf with other factors such as Pax6, Six3 and Prox1 had little or no effect on delta-crystallin. We also observed that L-Maf and Sox2 cooperatively enhanced the transactivation of a reporter gene bearing the delta-crystallin enhancer in ovo, implying that L-Maf and Sox2 can induce delta-crystallin through the same enhancer. In conclusion, we report here that L-Maf and Sox2 cooperatively regulate the expression of delta-crystallin during chick lens development.

摘要

晶状体发育受多种具有不同特性的转录因子调控。晶状体特异性转录因子L-Maf对晶状体形成至关重要,并诱导晶状体特异性标志物,如晶状体蛋白基因。在本研究中,我们分析了L-Maf调控δ-晶状体蛋白表达的机制。通过卵内电穿孔在晶状体板形成胚胎的头部外胚层中错误表达L-Maf,仅在晶状体周围区域诱导了δ-晶状体蛋白。为了确定这种受限表达,我们将L-Maf与其他参与δ-晶状体蛋白表达的转录因子一起错误表达。Sox2加L-Maf将δ-晶状体蛋白诱导域扩展到整个头部外胚层,并同时增加了所表达的δ-晶状体蛋白mRNA的量。相比之下,L-Maf与其他因子如Pax6、Six3和Prox1共表达对δ-晶状体蛋白几乎没有影响。我们还观察到L-Maf和Sox2在卵内协同增强了带有δ-晶状体蛋白增强子的报告基因的反式激活,这意味着L-Maf和Sox2可以通过相同的增强子诱导δ-晶状体蛋白。总之,我们在此报告,在鸡晶状体发育过程中,L-Maf和Sox2协同调节δ-晶状体蛋白的表达。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验