Andres Anne-Catherine, Munarini Nadia, Djonov Valentin, Bruneau Salomé, Zuercher Gisela, Loercher Saemi, Rohrbach Valeria, Ziemiecki Andrew
The Tiefenau Laboratory, Department of Clinical Research, University of Berne, Tiefenaustrasse 120, CH-3004 Berne, Switzerland.
Mech Dev. 2003 Apr;120(4):511-6. doi: 10.1016/s0925-4773(02)00461-6.
We have established transgenic mice over-expressing the EphB4 receptor tyrosine kinase in the kidney. The EphB4 protein was localised to the developing tubular system of both control and transgenic newborn mice. In transgenic adults, transgene expression persisted in the proximal tubules and the Bowman's capsules, structures, which were not stained in control kidneys. The glomeruli of control animals consisted of regular, round vascular baskets with clearly discernable afferent and efferent arterioles. In contrast, approximately 40% of the transgenic glomeruli had an irregular shrivelled appearance and many exhibited fused, horse shoe-like afferent and efferent arterioles bypassing the glomerulus. These abnormal glomerular structures are very reminiscent of aglomerular vascular shunts, a human degenerative glomerulopathy of unknown aetiology.
我们已经建立了在肾脏中过表达EphB4受体酪氨酸激酶的转基因小鼠。EphB4蛋白定位于对照和转基因新生小鼠发育中的肾小管系统。在转基因成年小鼠中，转基因表达在近端小管和鲍曼囊中持续存在，而对照肾脏中的这些结构未被染色。对照动物的肾小球由规则的圆形血管篮组成，具有清晰可辨的入球小动脉和出球小动脉。相比之下，约40%的转基因肾小球外观不规则且皱缩，许多表现出融合的马蹄形入球小动脉和出球小动脉绕过肾小球。这些异常的肾小球结构非常类似于无肾小球血管分流，一种病因不明的人类退行性肾小球病。
