KCNK9钾通道基因的基因组扩增及致癌特性

Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene.

作者信息

Mu David, Chen Liyun, Zhang Xiping, See Lei-Hoon, Koch Christina M, Yen Clifford, Tong James Jiayuan, Spiegel Lori, Nguyen Ken C Q, Servoss Allyson, Peng Yue, Pei Lin, Marks Jeffrey R, Lowe Scott, Hoey Timothy, Jan Lily Yeh, McCombie W Richard, Wigler Michael H, Powers Scott

机构信息

Tularik Inc, Genomics Division, 266 E Pulaski Road, Greenlawn, NY 11740, USA.

出版信息

Cancer Cell. 2003 Mar;3(3):297-302. doi: 10.1016/s1535-6108(03)00054-0.

DOI:10.1016/s1535-6108(03)00054-0

PMID:12676587

Abstract

Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.

摘要

利用人乳腺癌的代表性差异分析（RDA）来发现位于染色体区域8q24.3的一个新的扩增子。我们检测了一系列该区域存在扩增的乳腺癌样本，并确定KCNK9是扩增中心内唯一过度表达的基因。KCNK9编码一种钾通道，在10%的乳腺肿瘤中扩增3至10倍，在44%的乳腺肿瘤中过度表达5至100倍以上。KCNK9在细胞系中的过度表达促进肿瘤形成，并赋予对缺氧和血清剥夺的抗性，表明其扩增和过度表达在人类乳腺癌中发挥着重要的生理作用。