van Staden J F, Mulaudzi L V, Stefan R I
Department of Chemistry, University of Pretoria, 0002, Pretoria, South Africa.
Anal Bioanal Chem. 2003 Apr;375(8):1074-82. doi: 10.1007/s00216-003-1814-4. Epub 2003 Apr 3.
An on-line procedure for the simultaneous determination of bromine and total bromine (bromine+bromide oxidised to bromine) is proposed, which lead to the determination of bromide by subtraction. Phenol red was used as chromogenic reagent for bromine and total bromine after bromide was oxidised to bromine by Chloramine T. The linear range found is 1-10 mg L(-1) with a detection limit of 0.6 mg L(-1) for bromine, and a linear range of 0.8-15 mg L(-1) with a detection limit of 0.4 mg L(-1) for total bromine. The calculated RSD for bromine is less than 0.8% and for total bromine less than 0.7%. The system is fully computerised and able to run 30 samples per hour with an automated rinsing step that eliminates sample carry-over. The results for both bromine and bromide from the proposed sequential injection analysis (SIA) system compare favourably with standard manual methods and statistical evaluation proves no significant difference between the results of the proposed SIA system and the standard method at the 95% confidence level. The presence of other halides was found not to interfere.
提出了一种同时测定溴和总溴（溴 + 氧化为溴的溴化物）的在线程序，通过减法可测定溴化物。在溴化物被氯胺T氧化为溴后，酚红用作溴和总溴的显色剂。测得溴的线性范围为1 - 10 mg L⁻¹，检测限为0.6 mg L⁻¹；总溴的线性范围为0.8 - 15 mg L⁻¹，检测限为0.4 mg L⁻¹。计算得出溴的相对标准偏差小于0.8%，总溴的相对标准偏差小于0.7%。该系统完全计算机化，能够每小时运行30个样品，并带有自动冲洗步骤以消除样品残留。所提出的顺序注射分析（SIA）系统测定溴和溴化物的结果与标准手动方法相比具有优势，统计评估证明在所提出的SIA系统和标准方法的结果之间，在95%置信水平下无显著差异。发现其他卤化物的存在不会产生干扰。
