头孢唑啉/替考拉宁与头孢唑啉/万古霉素对耐甲氧西林金黄色葡萄球菌的体外联合效应

In vitro combined effects of cefozopran/teicoplanin and cefozopran/vancomycin on methicillin-resistant Staphylococcus aureus.

作者信息

Toyokawa M, Asari S, Nishi I, Horikawa M, Tsukamoto H, Sunada A, Ueda A, Iwatani Y

机构信息

Laboratory for Clinical Investigation, Osaka University Hospital, Osaka University, Suita City, Osaka, Japan.

出版信息

J Chemother. 2003 Feb;15(1):31-6. doi: 10.1179/joc.2003.15.1.31.

DOI:10.1179/joc.2003.15.1.31

PMID:12678411

Abstract

We determined the in vitro effects of teicoplanin (TEIC) or vancomycin (VCM) added to cefozopran (CZOP) on 50 methicillin-resistant Staphylococcus aureus (MRSA) strains, using a modified checkerboard method with serial 1.25-fold dilutions, and assessed the time-kill curve. CZOP + TEIC was synergistic (fractional inhibitory concentration index < or = 0.5) against 98% and CZOP + VCM against 20% of strains. Both drug combinations were additive against the remaining strains. A comparison of the fractional bactericidal concentration indices for 32 strains showed synergistic bactericidal effects for CZOP + TEIC in 88%, and in 48% for CZOP +VCM, confirming that CZOP + TEIC is superior to CZOP + VCM. The time-kill curve confirms that the bactericidal potency of these drugs is increased through combined use with CZOP. These results suggest that treatment using TEIC or VCM with CZOP may be useful in treating MRSA infections, including polymicrobial infections and those involving Gram-negative rods.

摘要

我们采用改良棋盘法（系列1.25倍稀释），测定了添加替考拉宁（TEIC）或万古霉素（VCM）的头孢唑肟（CZOP）对50株耐甲氧西林金黄色葡萄球菌（MRSA）菌株的体外作用，并评估了时间杀菌曲线。CZOP + TEIC对98%的菌株具有协同作用（部分抑菌浓度指数≤0.5），而CZOP + VCM对20%的菌株具有协同作用。两种药物组合对其余菌株均为相加作用。对32株菌株的部分杀菌浓度指数进行比较，结果显示CZOP + TEIC对88%的菌株具有协同杀菌作用，而CZOP + VCM对48%的菌株具有协同杀菌作用，证实CZOP + TEIC优于CZOP + VCM。时间杀菌曲线证实，这些药物与CZOP联合使用可增强杀菌效力。这些结果表明，TEIC或VCM与CZOP联合治疗可能有助于治疗MRSA感染，包括混合感染和涉及革兰氏阴性杆菌的感染。