Lu Guo-Zhong, Zhu Yu-Gang, Zhou Hong-Mei
Department of Burns, The Third People's Hospital, Wuxi 214041, Jiangsu Province, P.R. China.
Zhonghua Shao Shang Za Zhi. 2003 Feb;19(1):29-31.
To explore a method for in vivo skin reconstruction.
Thirty Sprague-Dawley (SD) rats were randomly divided into two paired groups, i.e. in vivo culture (A group, 10 pairs) and in vitro grafting (B group, 5 pairs). Skin samples were harvested from the rats of the two groups for the isolation of epithelial cells which were then mixed cultured in vitro in 1:1 ratio. Mixed cellular suspension in A group was harvested 4 days after culture. The mixed cellular sheets were harvested 14 days after culture. The cultured cells and sheets were then transplanted onto total skin loss wounds of donor rats for further cultivation. The wounds in A group were covered with allogeneic full-thickness skin. While the wounds in B group were covered by collagen membrane and gauze. Wound repair was observed and compared between the two groups at 2 - 3 post-operative weeks.
Most of the wounds in A group healed after 2 - 3 weeks with smooth surface, and the peithelium connected closely and tightly with the subcutaneous tissue. In the wounds in B group on 5 post-operative day, some of the cellular sheets survived and some fell off. Even the healed wounds in B group would be injured again resulting in protracted small wounds.
In vivo in situ epithelial culture might be an optional method of skin reconstruction for wound healing.
探索一种体内皮肤重建的方法。
将30只Sprague-Dawley（SD）大鼠随机分为两组，即体内培养组（A组，10对）和体外移植组（B组，5对）。从两组大鼠身上采集皮肤样本以分离上皮细胞，然后将其按1:1比例进行体外混合培养。A组在培养4天后收集混合细胞悬液。在培养14天后收集混合细胞片。然后将培养的细胞和细胞片移植到供体大鼠的全层皮肤缺损伤口上进行进一步培养。A组伤口用同种异体全层皮肤覆盖。而B组伤口用胶原膜和纱布覆盖。在术后2至3周观察并比较两组的伤口修复情况。
A组大部分伤口在2至3周后愈合，表面光滑，上皮与皮下组织紧密相连。在术后第5天的B组伤口中，一些细胞片存活，一些脱落。即使B组愈合的伤口也会再次受伤，导致伤口迁延不愈。
体内原位上皮培养可能是伤口愈合皮肤重建的一种可选方法。
