Xia Zhi-bai, Pu Pei-yu, Huang Qiang, You Yong-ping, Wang Guang-xiu, Wang Chun-yan
Department of Neurosurgery, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Zhonghua Zhong Liu Za Zhi. 2003 Jan;25(1):4-8.
To study the mechanism involved in the control of glioma cell proliferation with transfection of connexin (Cx) 43 gene.
C6 rat glioma and TJ905 human glioblastoma cell lines without Cx43 gene expression were transfected with Cx43cDNA mediated by lipofectamine. Northern blot, in situ hybridization and immunohistochemical technology were used to detect the expression of Cx43mRNA and its protein with MTT assay and silver colloid stain for the detection of cell proliferation, TUNEL method for determination of cell apoptosis, scrape loading dye transfer (SLDT) for GJIC, Western blot and immunohistochemical technology for bFGF, PDGF, EGFR, IGF-I and IGFBP3 expression.
Cx 43 gene transfected glioma cells showed decreased proliferation, restored GJIC and decreased bFGF, PDGF, IGFBP3, except EGFR expression and cell apoptosis which showed no change.
The mechanism of Cx 43 gene inhibiting gliomas cell proliferation is the restoration of GJIC and decreased autocrine growth factors.
研究转染连接蛋白（Cx）43基因对胶质瘤细胞增殖调控的机制。
用脂质体介导的Cx43 cDNA转染无Cx43基因表达的C6大鼠胶质瘤细胞系和TJ905人胶质母细胞瘤细胞系。采用Northern印迹、原位杂交和免疫组化技术检测Cx43mRNA及其蛋白的表达，用MTT法和银胶体染色检测细胞增殖，用TUNEL法检测细胞凋亡，用刮擦加载染料转移（SLDT）法检测缝隙连接细胞间通讯（GJIC），用Western印迹和免疫组化技术检测碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子（PDGF）、表皮生长因子受体（EGFR）、胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子结合蛋白3（IGFBP3）的表达。
转染Cx 43基因的胶质瘤细胞增殖减少，GJIC恢复，bFGF、PDGF、IGFBP3表达降低，但EGFR表达及细胞凋亡无变化。
Cx 43基因抑制胶质瘤细胞增殖的机制是GJIC的恢复和自分泌生长因子的减少。
