[检测胃癌和结直肠癌患者外周血中播散肿瘤细胞的意义]

[Significance of detecting disseminated tumor cells in peripheral blood of gastric and colorectal cancer patients].

作者信息

Zhang Xi-wei, Fan Ping, Yang Hong-yu, Yang Li, Chen Guo-yu

机构信息

Department of General Surgery, First Hospital, Nanjing Medical University, Nanjing 210029, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2003 Jan;25(1):66-9.

PMID:12678992

Abstract

OBJECTIVE

To evaluate the clinical significance of CK20 mRNA expression in detecting disseminated tumor cells in peripheral blood of gastric and colorectal cancer patients.

METHODS

Expression of CK20 mRNA was investigated by RT-PCR in bone marrow, portal vein and peripheral blood in 47 gastric, 58 colorectal cancer patients and 6 non-cancer volunteers. All the patients were followed-up for one year.

RESULTS

There was no positive expression of CK20 mRNA in 6 non-cancer volunteers. The positive rates of CK20 mRNA in bone marrow, portal vein were 87.2% (41/47) and 85.1% (40/47) in gastric cancer, and were 77.6% (45/58) and 74.1% (43/58) in colorectal cancer. The positive rates of CK20 mRNA in peripheral blood in gastric and colorectal cancer patients were 42.6% (20/47) and 44.8% (26/58) by one single test, and were 74.5% (35/47) and 69.0% (40/58) by two tests. The overall positive rate of CK20 mRNA in peripheral blood (two tests) was similar to that in bone marrow and portal vein. The overall positive rate of CK20 mRNA in peripheral blood was higher in two tests than in one single test (P < 0.05) and in advanced than early lesions. The relapse rate within one year was higher in CK20 mRNA positive patients than the negative ones (P < 0.05).

CONCLUSION

Detection of cancer cells by RT-PCR for CK20 mRNA in peripheral blood, being as sensitive and specific as in bone marrow and portal vein, is reliable and convenient in diagnosing micrometastasis of gastric and colorectal cancer, which possesses clinical significance in assessing the prognosis and scheme of therapy.

摘要

目的

评估细胞角蛋白20（CK20）mRNA表达在检测胃癌和结直肠癌患者外周血中播散肿瘤细胞的临床意义。

方法

采用逆转录聚合酶链反应（RT-PCR）检测47例胃癌、58例结直肠癌患者及6例非癌志愿者骨髓、门静脉和外周血中CK20 mRNA的表达。所有患者均随访1年。

结果

6例非癌志愿者CK20 mRNA均无阳性表达。胃癌患者骨髓、门静脉中CK20 mRNA阳性率分别为87.2%（41/47）和85.1%（40/47），结直肠癌患者分别为77.6%（45/58）和74.1%（43/58）。胃癌和结直肠癌患者外周血中CK20 mRNA单次检测阳性率分别为42.6%（20/47）和44.8%（26/58），两次检测阳性率分别为74.5%（35/47）和69.0%（40/58）。外周血中CK20 mRNA总体阳性率（两次检测）与骨髓和门静脉相似。外周血中CK20 mRNA总体阳性率两次检测高于单次检测（P<0.05），晚期病变高于早期病变。CK20 mRNA阳性患者1年内复发率高于阴性患者（P<0.05）。