Oude Elferink Ronald
AMC Liver Center, Academic Medical Center S2-162, Meibergdreef 69-71, Amsterdam, The Netherlands.
Trends Biotechnol. 2003 Apr;21(4):146-7; discussion 147-8. doi: 10.1016/S0167-7799(03)00029-5.
In a recent paper by Michiels et al. an important step was made towards genuine high throughput functional genomics. The authors produced an arrayed adenoviral library containing >120000 cDNAs isolated from human placenta. This library can be used for arrayed transduction of cell lines in phenotypic assays and to screen for genes involved in the induction of any phenotype for which a robust high-throughput assay can be designed.
在米歇尔斯等人最近发表的一篇论文中,朝着真正的高通量功能基因组学迈出了重要一步。作者构建了一个阵列腺病毒文库,其中包含从人胎盘中分离出的超过120000个cDNA。该文库可用于在表型分析中对细胞系进行阵列转导,并筛选参与诱导任何可设计出强大高通量分析方法的表型的基因。