Kim Hyun-Woo, Kwon Young-Bae, Ham Tae-Won, Roh Dae-Hyun, Yoon Seo-Yeon, Lee Hye-Jung, Han Ho-Jae, Yang Il-Suk, Beitz Alvin J, Lee Jang-Hern
Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
J Vet Med Sci. 2003 Mar;65(3):349-55. doi: 10.1292/jvms.65.349.
In two previous reports, we have demonstrated that injection of bee venom (BV) into an acupoint produces a significant antinociceptive and anti-inflammatory effect in both a mouse model of visceral nociception and a rat model of chronic arthritis. The present study was designed to evaluate the potential antinociceptive effect of BV pretreatment on formalin-induced pain behavior and it associated spinal cord Fos expression in rats. Adult Sprague-Dawley rats were injected with BV directly into the Zusanli (ST36) acupoint or into an arbitrary non-acupoint located on the back. BV pretreatment into the Zusanli acupoint significantly decreased paw-licking time in the late phase of the formalin test. In contrast, BV injected into a non-acupoint in the back region did not suppress the paw-licking time. In addition, BV pretreatment into the Zusanli acupoint markedly inhibited spinal cord Fos expression induced by formalin injection. These findings indicate that BV pretreatment into the Zusanli acupoint has an antinociceptive effect on formalin-induced pain behavior.
在之前的两份报告中，我们已经证明，将蜂毒（BV）注射到穴位中，在内脏痛觉过敏的小鼠模型和慢性关节炎的大鼠模型中均产生显著的抗伤害感受和抗炎作用。本研究旨在评估BV预处理对福尔马林诱导的大鼠疼痛行为及其相关脊髓Fos表达的潜在抗伤害感受作用。将成年Sprague-Dawley大鼠的足三里（ST36）穴位或背部任意非穴位直接注射BV。足三里穴位的BV预处理显著减少了福尔马林试验后期的舔爪时间。相比之下，注射到背部非穴位的BV并没有抑制舔爪时间。此外，足三里穴位的BV预处理显著抑制了福尔马林注射诱导的脊髓Fos表达。这些发现表明，足三里穴位的BV预处理对福尔马林诱导的疼痛行为具有抗伤害感受作用。
