雌激素受体及雌激素受体信使核糖核酸在胃癌组织中的表达
Expression of estrogen receptor and estrogen receptor messenger RNA in gastric carcinoma tissues.
作者信息
Zhao Xin-Han, Gu Shan-Zhi, Liu Shan-Xi, Pan Bo-Rong
机构信息
Department of Medical Oncology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2003 Apr;9(4):665-9. doi: 10.3748/wjg.v9.i4.665.
AIM
To study estrogen receptor (ER) and estrogen receptor messenger RNA (ERmRNA) expression in gastric carcinoma tissues and to investigate their association with the pathologic types of gastric carcinoma.
METHODS
The expression of ER and ERmRNA in gastric carcinoma tissues (15 males and 15 females, 42-70 years old) was detected by immunohistochemistry and in situ hybridization, respectively.
RESULTS
The positive rate of ER (immunohistochemistry) was 33.3 % in males and 46.7 % in females. In Borrmann IV gastric carcinoma ER positive rate was greater than that in other pathologic types, and in poorly differentiated adenocarcinoma and signet ring cell carcinoma the positive rates were greater than those in other histological types of both males and females (P<0.05). The ER was more highly expressed in diffused gastric carcinoma than in non-diffused gastric carcinoma (P<0.05). The ER positive rate was also related to regional lymph nodes metastases (P<0.05), and was significantly higher in females above 55 years old, and higher in males under 55 years old (P<0.05). The ERmRNA (in situ hybridization) positive rate was 73.3 % in males and 86.7 % in females. The ERmRNA positive rates were almost the same in Borrmann I, II, III and IV gastric carcinoma (P>0.05). ERmRNA was expressed in all tubular adenocarcinoma, poorly differentiated adenocarcinoma and signet ring cell carcinoma (P<0.05). The ERmRNA positive rate was related to both regional lymph nodes metastases and gastric carcinoma growth patterns, and was higher in both sexes above 55 years old but without statistical significance (P>0.05). The positive rate of ERmRNA expression by in situ hybridization was higher than that of ER expression by immunohistochemistry (P<0.05).
CONCLUSION
ERmRNA expression is related to the pathological behaviors of gastric carcinoma, which might help to predict the prognosis and predict the effectiveness of endocrine therapy for gastric carcinoma.
目的
研究雌激素受体（ER）及雌激素受体信使核糖核酸（ERmRNA）在胃癌组织中的表达情况，并探讨其与胃癌病理类型的关系。
方法
分别采用免疫组织化学和原位杂交方法检测胃癌组织（男性15例，女性15例，年龄42 - 70岁）中ER和ERmRNA的表达。
结果
ER（免疫组织化学）阳性率男性为33.3%，女性为46.7%。Borrmann Ⅳ型胃癌的ER阳性率高于其他病理类型，在低分化腺癌和印戒细胞癌中，男性和女性的阳性率均高于其他组织学类型（P<0.05）。弥漫型胃癌中ER的表达高于非弥漫型胃癌（P<0.05）。ER阳性率还与区域淋巴结转移有关（P<0.05），55岁以上女性显著高于55岁以下男性（P<0.05）。ERmRNA（原位杂交）阳性率男性为73.3%，女性为86.7%。Borrmann Ⅰ、Ⅱ、Ⅲ和Ⅳ型胃癌的ERmRNA阳性率几乎相同（P>0.05）。ERmRNA在所有管状腺癌、低分化腺癌和印戒细胞癌中均有表达（P<0.05）。ERmRNA阳性率与区域淋巴结转移及胃癌生长方式有关，55岁以上男女阳性率均较高，但无统计学意义（P>0.05）。原位杂交检测的ERmRNA表达阳性率高于免疫组织化学检测的ER表达阳性率（P<0.05）。
结论
ERmRNA表达与胃癌的病理行为有关，可能有助于预测胃癌预后及内分泌治疗效果。
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