Serini Guido, Ambrosi Davide, Giraudo Enrico, Gamba Andrea, Preziosi Luigi, Bussolino Federico
Institute for Cancer Research and Treatment and Department of Oncological Sciences, University of Torino, 10060 Candiolo (TO).
EMBO J. 2003 Apr 15;22(8):1771-9. doi: 10.1093/emboj/cdg176.
In vertebrates, networks of capillary vessels supply tissues with nutrients. Capillary patterns are closely mimicked by endothelial cells cultured on basement membrane proteins that allow single randomly dispersed cells to self-organize into vascular networks. Here we provide a model including chemoattraction as the fundamental mechanism for cell-to-cell communication in order to identify key parameters in the complexity of the formation of vascular patterns. By flanking biological experiments, theoretical insights and numerical simulations, we provide strong evidence that endothelial cell number and the range of activity of a chemoattractant factor regulate vascular network formation and size. We propose a mechanism linking the scale of formed endothelial structures to the range of cell-to-cell interaction mediated by the release of chemoattractants.
在脊椎动物中，毛细血管网络为组织提供营养。在基底膜蛋白上培养的内皮细胞能紧密模拟毛细血管模式，这些基底膜蛋白可使单个随机分散的细胞自组织形成血管网络。在此，我们提供了一个模型，该模型将化学吸引作为细胞间通讯的基本机制，以确定血管模式形成复杂性中的关键参数。通过结合生物学实验、理论见解和数值模拟，我们提供了有力证据，证明内皮细胞数量和化学吸引因子的活性范围调节血管网络的形成和大小。我们提出了一种机制，将形成的内皮结构的规模与由化学吸引剂释放介导的细胞间相互作用范围联系起来。
