Cieślikowski Tomasz, Gradecka Dobrosława, Mielczarek Magdalena, Kaca Wiesław
Centre of Microbiology and Virology, Polish Academy of Sciences, Łódź, Poland.
J Clin Microbiol. 2003 Apr;41(4):1673-80. doi: 10.1128/JCM.41.4.1673-1680.2003.
Two microsatellite tandem repeated tetramers, (GACA)(4) and (CAAT)(4), were used for Proteus mirabilis strain differentiation. The microsatellite-based PCR tests were applied for the examination of interstrain diversity for 87 P. mirabilis strains. Forty-six of the investigated strains were clinical isolates (5 were hospital isolates and 39 were outpatient clinic isolates); 42 strains were derived from the Kauffmann-Perch collection of laboratory strains. Fingerprinting done with the tetramers had a high discrimination ability [0.992 and 0.940 for (GACA)(4) and (CAAT)(4), respectively]. The distributions of clinical isolates among well-defined laboratory strains, determined by numerical analysis (unweighted pair-group method with arithmetic averages; Dice similarity coefficient), proved their genetic similarity to reference strains in the Kauffmann-Perch collection. This analysis also indicated that it is possible to estimate some phenotypic properties of P. mirabilis clinical isolates solely on the basis of microsatellite fingerprinting.
两个微卫星串联重复四聚体，(GACA)(4) 和 (CAAT)(4)，用于奇异变形杆菌菌株的鉴别。基于微卫星的聚合酶链反应（PCR）测试用于检测87株奇异变形杆菌菌株间的多样性。其中46株被调查菌株为临床分离株（5株为医院分离株，39株为门诊分离株）；42株菌株来源于考夫曼-佩奇实验室菌株库。用这些四聚体进行指纹图谱分析具有很高的鉴别能力[对于(GACA)(4)和(CAAT)(4)分别为0.992和0.940]。通过数值分析（算术平均的非加权配对组法；戴斯相似系数）确定的临床分离株在明确的实验室菌株中的分布，证明了它们与考夫曼-佩奇菌株库中参考菌株的遗传相似性。该分析还表明，仅基于微卫星指纹图谱就有可能估计奇异变形杆菌临床分离株的一些表型特性。
