Kind T V
Laboratory of Entomology, Biological Institute of St. Petersburg State University.
Tsitologiia. 2003;45(1):14-25.
On the basis of in vitro observation of live cells and examination of stained slides of larval and prepupal Calliphora vicina hemolymph, seven types of hemocytes have been detected: prohemocytes, stable and unstable hyaline cells, thrombocytoids, spindle cells, larval plasmatocytes, and plasmatocytes I-IV, a. The last representing sequential stages of one cell line differentiation. Prohemocytes are basic cells, from which other forms of hemocytes derive outside the hemopoietic tissue, i.e. in free hemolymph. At the last larval instar, three waves of hemopoiesis occur. Either wave tends to increase the general number of cells and to change the quality of hemocyte population. The first wave occurs at the close of larva feeding and is accompanied by increase in the number of hyaline hemocytes, thrombocytoids and larval plasmatocytes. The second wave of hemopoiesis occurs after the larva's crop emptying. In this period the main increase of hemocyte population occurs at the expense of prohemocytes and plasmatocytes I. The most significant (five-fold) explosion of the population of free hemocytes takes place at the onset of pupariation and correlates with the rise of ecdysone titer. At the first stage of this peak, the amount of plasmatocytes I sharply increases. Further on these are rapidly differentiated into plasmatocytes II and III. After the puparium formation, hemocytes are reduced in number. Plasmatocytes III phagocytose fragments of destroyed larval tissues, pass to the stage of plasmatocytes IV (macrophages), and partially settle on tissues.
基于对活细胞的体外观察以及对幼虫和蛹前期红头丽蝇血淋巴染色玻片的检查，已检测到七种血细胞类型：原血细胞、稳定和不稳定的透明细胞、类血小板、纺锤细胞、幼虫浆细胞以及I-IV型浆细胞a。最后一种代表一个细胞系分化的连续阶段。原血细胞是基础细胞，其他形式的血细胞在造血组织之外，即在游离血淋巴中由其衍生而来。在幼虫最后一龄期，发生三波血细胞生成。每一波都倾向于增加细胞总数并改变血细胞群体的质量。第一波发生在幼虫进食结束时，伴随着透明血细胞、类血小板和幼虫浆细胞数量的增加。第二波血细胞生成发生在幼虫嗉囊排空之后。在此期间，血细胞群体的主要增加是以原血细胞和I型浆细胞为代价的。游离血细胞群体最显著（五倍）的激增发生在化蛹开始时，并且与蜕皮激素滴度的升高相关。在这个峰值的第一阶段，I型浆细胞的数量急剧增加。在此之后，它们迅速分化为II型和III型浆细胞。蛹形成后，血细胞数量减少。III型浆细胞吞噬被破坏的幼虫组织碎片，进入IV型浆细胞（巨噬细胞）阶段，并部分定居在组织上。
