Sun Xiaoxi, Christow Alexander, Marions Lena, Gemzell-Danielsson Kristina
Department of Woman and Child Health, Division for Obstetrics and Gynecology, Karolinska Hospital/Institute, S-171 76, Stockholm, Sweden.
Contraception. 2003 Apr;67(4):319-26. doi: 10.1016/s0010-7824(02)00513-9.
The distribution of progesterone receptor isoform B may have important clinical significance. The aim of this study was to compare the expression, localization and regulation of progesterone receptor isoform B in the human Fallopian tube and endometrium following mifepristone in a dose effective for contraception. Fertile women were treated with a single dose of 200 mg mifepristone on day luteinizing hormone (LH)+2. Biopsies were obtained from the Fallopian tube on day LH+4 to LH+6 and from the endometrium on day LH+6 to LH+8. Progesterone receptor isoform B expression was analyzed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Treatment with mifepristone increased progesterone receptor isoform B concentration in epithelial and stromal cells in the Fallopian tube and also increased progesterone receptor isoform B concentration in the glandular cells of the endometrium. These results further support the hypothesis that the contraceptive effect of mifepristone when given postovulatory is primarily due to alteration of the peri-implantation milieu influencing endometrial receptivity.
孕激素受体B亚型的分布可能具有重要的临床意义。本研究的目的是比较米非司酮以有效避孕剂量给药后，人输卵管和子宫内膜中孕激素受体B亚型的表达、定位及调节情况。在黄体生成素（LH）+2日，对有生育能力的女性给予单剂量200mg米非司酮。在LH+4日至LH+6日从输卵管获取活检组织，在LH+6日至LH+8日从子宫内膜获取活检组织。通过免疫组织化学和逆转录聚合酶链反应分析孕激素受体B亚型的表达。米非司酮治疗增加了输卵管上皮和基质细胞中孕激素受体B亚型的浓度，也增加了子宫内膜腺细胞中孕激素受体B亚型的浓度。这些结果进一步支持了以下假说：排卵后给予米非司酮的避孕作用主要是由于影响子宫内膜容受性的着床周围环境的改变。
