Wang Zhiyu, Yao Ping, Song Yanyan, Wang Guiting, Wang Yongkang, Xu Hongzhi, Wang Peng
Department of Virology, School of Public Health, Shandong University, Jinan, People's Republic of China.
Intervirology. 2003;46(2):79-85. doi: 10.1159/000069742.
OBJECTIVE: To investigate the correlation between rubella virus (RuV) antigen in peripheral lymphocytes, the immune status and RuV infection in the central nervous system (CNS). METHODS: BALB/c mice were used as a model and treated with immunoaffecting medicines. Then, the mice were infected with RuV via the abdominal cavity, and the antigen level in peripheral lymphocytes was examined 1, 3, 7 and 14 days postinfection. RuV in the CNS was detected by immunohistochemical methods. BALB/c mice were given dexamethasone and cytoxan before infection with the RuV JR23 strain. Immune functions and RuV invasion of the CNS were assayed on day 21 postinfection via the abdominal cavity, and their relationship was analyzed. RESULTS: The mean antigen detection rates at different times were 3.1, 4.1, 9.6 and 2.4%, respectively, in the dexamethasone group, and 14.2, 12.7, 9.9 and 3.1%, respectively, in the cytoxan group. In the group without any intervention, the detection rates were 4.63, 10.25, 6.88 and 1.75%, respectively. The antigen detection rates in peripheral lymphocytes among the three groups 24 h postinfection were significantly different (F = 0.0317, p < 0.05). Comparisons between groups showed that antigen detection rates in the cytoxan group were much higher than those in other groups, but there was no difference between the dexamethasone and control groups. The animals with persistent presence of antigen were much more susceptible to cerebral infection than those with short-term presence (p < 0.001). T cell functions of the cytoxan group were significantly lower than those of other groups (p < 0.05), as detected by the MTT method. Infection rates of the dexamethasone, cytoxan and control groups were 60, 90 and 50%, respectively. Cell immune functions of the mice with CNS infection were obviously worse than those of the mice without CNS infection (p < 0.001). RuV-specific antibodies were assayed in all groups by ELISA and the results showed that there were no significant differences among groups (p < 0.05) or between the groups with and without CNS infection. CONCLUSION: Cytoxan can affect virus detection rates in peripheral lymphocytes. At the early phase of infection, the persistent presence of RuV in peripheral lymphocytes increases the incidence of CNS infection. RuV infection in the CNS was related to the cell immune situation before specific antibody was produced in the body.
目的：探讨外周淋巴细胞中风疹病毒（RuV）抗原、免疫状态与中枢神经系统（CNS）风疹病毒感染之间的相关性。 方法：以BALB/c小鼠为模型，给予免疫影响药物处理。然后经腹腔感染RuV，于感染后1、3、7和14天检测外周淋巴细胞中的抗原水平。采用免疫组化方法检测CNS中的RuV。在感染RuV JR23株前，给BALB/c小鼠注射地塞米松和环磷酰胺。于感染后第21天经腹腔检测免疫功能和RuV对CNS的侵袭情况，并分析它们之间的关系。 结果：地塞米松组不同时间的平均抗原检测率分别为3.1%、4.1%、9.6%和2.4%，环磷酰胺组分别为14.2%、12.7%、9.9%和3.1%。在未进行任何干预的组中，检测率分别为4.63%、10.25%、6.88%和1.75%。感染后24小时，三组外周淋巴细胞中的抗原检测率有显著差异（F = 0.0317，p < 0.05）。组间比较显示，环磷酰胺组的抗原检测率远高于其他组，但地塞米松组与对照组之间无差异。抗原持续存在的动物比抗原短期存在的动物更易发生脑部感染（p < 0.001）。采用MTT法检测，环磷酰胺组的T细胞功能显著低于其他组（p < 0.05）。地塞米松组、环磷酰胺组和对照组的感染率分别为60%、90%和50%。CNS感染小鼠的细胞免疫功能明显低于未感染CNS的小鼠（p < 0.001）。通过ELISA检测所有组中的RuV特异性抗体，结果显示组间（p < 0.05）或有无CNS感染的组间均无显著差异。 结论：环磷酰胺可影响外周淋巴细胞中的病毒检测率。在感染早期，外周淋巴细胞中RuV的持续存在会增加CNS感染的发生率。CNS中的RuV感染与机体产生特异性抗体之前的细胞免疫状况有关。
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