用于检测风疹病毒抗原的双抗体夹心酶联免疫吸附测定
Double antibody sandwich ELISA for the detection of rubella virus antigen.
作者信息
Komatsu H, Suzuki Y, Matumoto M
机构信息
Department of Virology, Kitasato Institute, Tokyo, Japan.
出版信息
Kitasato Arch Exp Med. 1992 Apr;65(1):23-31.
We developed enzyme linked immunosorbent assay (ELISA) for the detection of rubella virus antigen, using two monoclonal antibodies, MC-7 and MC-22. The double antibody sandwich ELISA method was carefully standardized and found to be sensitive enough to detect as small as 2.5 ng protein of rubella virus. The infective titers by the double antibody sandwich ELISA closely related to those judged by interference of vesicular stomatitis virus in RK-13 cells. The method is simple, sensitive, and readily applicable to the detection of rubella virus.
我们使用两种单克隆抗体MC - 7和MC - 22开发了用于检测风疹病毒抗原的酶联免疫吸附测定（ELISA）。对双抗体夹心ELISA方法进行了仔细的标准化，发现其灵敏度足以检测低至2.5 ng的风疹病毒蛋白。双抗体夹心ELISA的感染滴度与通过水疱性口炎病毒在RK - 13细胞中的干扰判断的滴度密切相关。该方法简单、灵敏，易于应用于风疹病毒的检测。
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