[木瓜凝乳蛋白酶兔抗体的制备与鉴定]
[The preparation and identification of rabbit's antibody of chymopapain].
作者信息
Wang Sufang, Yang Taicheng, Jiang Linlan, Zhao Shujin
机构信息
General Hospital of Guangzhou Military District, Guangzhou, 510010.
出版信息
Zhong Yao Cai. 2002 Dec;25(12):891-3.
PMID:12685352
Abstract
Chymopapain, one of the four cysteine proteinases of papaya latex, has milk clotting and proteolytic activity. It is mainly used to treat prolapsed intervertebral discs. This paper introduced the preparation of polyclonal antibody of partially purified chymopapain and the identification of the antibody specificity. The polyclonal antibody provides a basis for the further researches and applications of chymopapain.
摘要
木瓜凝乳蛋白酶是番木瓜乳胶中的四种半胱氨酸蛋白酶之一，具有凝乳和蛋白水解活性。它主要用于治疗椎间盘突出症。本文介绍了部分纯化的木瓜凝乳蛋白酶多克隆抗体的制备及其抗体特异性鉴定。该多克隆抗体为木瓜凝乳蛋白酶的进一步研究和应用提供了依据。
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