Manetto Giulia, Silvana Bellini Maria, Deyl Zdenek
GlaxoSmithKline, Department of Pharmacology and Analytical Chemistry, Verona, Italy.
J Chromatogr A. 2003 Mar 21;990(1-2):281-9. doi: 10.1016/s0021-9673(03)00056-6.
Liposomes can be effectively deposited on the inner surface of a capillary wall by flushing the electrophoretic system with a liposome suspension followed by air-drying of the capillary and removal of the excess of loosely bound liposomes by a 0.1 M NaOH wash. It was demonstrated that capillaries prepared in this way could be used for studies of analyte (drug)-liposome binding. The results were expressed as free binding energy changes [delta(deltaG0)] relatively to an arbitrarily selected standard (acetylsalicylic acid). The results were compared to [delta(deltaG0)] changes obtained from binding studies effected by capillary electrophoresis using a stable liposome plug in a capillary with minimized endoosmotic flow. Good agreement of data reported in the literature (without correction for the residual endoosmotic flow), our previous data obtained in a similar way (however, after the correction for the residual endoosmotic flow) and data obtained by the immobilized liposome affinity electrochromatography reported in this communication was achieved.
通过用脂质体悬浮液冲洗电泳系统，然后将毛细管风干，并通过0.1 M NaOH洗涤去除过量的松散结合的脂质体，脂质体可以有效地沉积在毛细管壁的内表面上。结果表明，以这种方式制备的毛细管可用于研究分析物（药物）-脂质体结合。结果以相对于任意选定标准（乙酰水杨酸）的自由结合能变化[Δ（ΔG0）]表示。将结果与通过在具有最小化内渗流的毛细管中使用稳定的脂质体塞进行毛细管电泳结合研究获得的[Δ（ΔG0）]变化进行比较。本通讯中报道的文献数据（未校正残余内渗流）、我们以类似方式获得的先前数据（然而，在校正残余内渗流之后）以及通过固定化脂质体亲和电色谱获得的数据之间达成了良好的一致性。
