Nagy Dusty W, Tyler Jeff W, Kleiboeker Steven B, Stoker Aaron
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
J Am Vet Med Assoc. 2003 Apr 1;222(7):983-5. doi: 10.2460/javma.2003.222.983.
To evaluate the use of a polymerase chain reaction (PCR) assay in detecting bovine leukosis virus (BLV) in adult dairy cows.
Prospective study.
223 adult dairy cows.
Cows were tested for BLV status by use of an ELISA and a PCR assay. Sensitivity, specificity, predictive values of positive and negative tests, and the percentage of cows correctly classified by PCR assay were calculated. Ninety-five percent confidence intervals were calculated for sensitivity and specificity.
Sensitivity and specificity were 0.672 and 1.00, respectively. Prevalence of BLV in this herd was 0.807. Predictive value of a positive test was 1.00, and predictive value of a negative test was 0.421. The percentage of cows correctly classified by PCR assay was 73.5%.
A positive PCR assay result provided definitive evidence that a cow was infected with BLV. Sensitivity and negative predictive value for PCR assay were low. Consequently, PCR assay alone is unreliable for routine detection of BLV in herds with high prevalence of the disease.
评估聚合酶链反应（PCR）检测法在检测成年奶牛牛白血病病毒（BLV）中的应用。
前瞻性研究。
223头成年奶牛。
使用酶联免疫吸附测定（ELISA）和PCR检测法对奶牛的BLV感染状况进行检测。计算敏感性、特异性、阳性和阴性检测的预测值，以及通过PCR检测法正确分类的奶牛百分比。计算敏感性和特异性的95%置信区间。
敏感性和特异性分别为0.672和1.00。该牛群中BLV的流行率为0.807。阳性检测的预测值为1.00，阴性检测的预测值为0.421。通过PCR检测法正确分类的奶牛百分比为73.5%。
PCR检测法呈阳性结果可提供确凿证据证明奶牛感染了BLV。PCR检测法的敏感性和阴性预测值较低。因此，对于该疾病高流行率的牛群，仅靠PCR检测法进行BLV的常规检测并不可靠。
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