Kalogeris E, Iniotaki F, Topakas E, Christakopoulos P, Kekos D, Macris B J
Biosystems Technology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Strasse, Zografou Campus, Athens 15 700, Greece.
Bioresour Technol. 2003 Feb;86(3):207-13. doi: 10.1016/s0960-8524(02)00175-x.
A laboratory bioreactor, designed for solid-state fermentation of thermophilic microorganisms, was operated for production of cellulases and hemicellulases by the thermophilic fungus Thermoascus aurantiacus. The suitability of the apparatus for the effective control of important operating variables affecting growth of microbes in solid-state cultivation was determined. Application of the optimum conditions found for the moisture content of the medium, growth temperature and airflow rate produced enzyme yields of 1709 U endoglucanase, 4 U cellobiohydrolase, 79 U beta-glucosidase, 5.5 U FPA, 4490 U xylanase and 45 U beta-xylosidase per g of dry wheat straw. The correlation between microorganism growth and production of enzymes was efficiently described by the Le Duy kinetic model.
一种为嗜热微生物固态发酵设计的实验室生物反应器，用于嗜热真菌橙色嗜热子囊菌生产纤维素酶和半纤维素酶。确定了该装置对有效控制影响固态培养中微生物生长的重要操作变量的适用性。将为培养基水分含量、生长温度和气流速率找到的最佳条件应用于生产，每克干麦秸产生的酶产量为：内切葡聚糖酶1709 U、纤维二糖水解酶4 U、β-葡萄糖苷酶79 U、滤纸酶活力5.5 U、木聚糖酶4490 U和β-木糖苷酶45 U。Le Duy动力学模型有效地描述了微生物生长与酶产生之间的相关性。
