Enzymatic profiling system in a small-molecule microarray.
作者信息
Zhu Qing, Uttamchandani Mahesh, Li Dongbo, Lesaicherre Marie L, Yao Shao Q
机构信息
Department of Chemistry, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
出版信息
Org Lett. 2003 Apr 17;5(8):1257-60. doi: 10.1021/ol034233h.
PMID:12688733
Abstract
[reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.
摘要
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