Kayasuga Ryoji, Iba Yoshinori, Hossen Maria Alejandra, Watanabe Takeshi, Kamei Chiaki
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.
Int Immunopharmacol. 2003 Apr;3(4):469-73. doi: 10.1016/S1567-5769(02)00254-0.
The involvement of chemical mediators other than histamine in eosinophil infiltration in the nasal mucosa was studied using histamine H(1) receptor-deficient mice. Histamine H(1) receptor-deficient mice and wild-type controls were immunized with ovalbumin and consecutive topical antigen instillation was performed. Histological alterations and eosinophil infiltration into the nasal mucosa of mice were examined. Diffuse infiltration of inflammatory cells and edema after sensitization with antigen were observed in the nasal mucosa in both wild-type and histamine H(1) receptor-deficient mice. The number of eosinophils in the nasal mucosa in mice sensitized with antigen was significantly increased as compared with controls. The number of eosinophils in the nasal mucosa was significantly decreased by cetirizine and epinastine, ramatroban and zafirlukast in wild-type mice. Not only histamine but also thromboxane A(2) and leukotrienes play important roles in allergic rhinitis, especially in the late phase participating in nasal eosinophilia.
利用组胺H(1)受体缺陷小鼠，研究了组胺以外的化学介质在鼻黏膜嗜酸性粒细胞浸润中的作用。将组胺H(1)受体缺陷小鼠和野生型对照用卵清蛋白免疫，并进行连续的局部抗原滴注。检查小鼠鼻黏膜的组织学改变和嗜酸性粒细胞浸润情况。在野生型和组胺H(1)受体缺陷小鼠的鼻黏膜中，均可观察到抗原致敏后炎症细胞的弥漫性浸润和水肿。与对照组相比，抗原致敏小鼠鼻黏膜中的嗜酸性粒细胞数量显著增加。在野生型小鼠中，西替利嗪、依匹斯汀、雷马曲班和扎鲁司特可使鼻黏膜中的嗜酸性粒细胞数量显著减少。不仅组胺，血栓素A(2)和白三烯在变应性鼻炎中也起重要作用，尤其是在参与鼻嗜酸性粒细胞增多的晚期阶段。
