Improved PCR-based method for the direct screening of Streptomyces transformants.
作者信息
Van Dessel W, Van Mellaert L, Geukens N, Anné J
机构信息
Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Louvain, Belgium.
出版信息
J Microbiol Methods. 2003 Jun;53(3):401-3. doi: 10.1016/s0167-7012(02)00235-x.
Abstract
Streptomycetes are attractive microorganisms because of their high secretion capacity and for the production of secondary metabolites. We report the improvement of a PCR-based method for screening of solid media-grown Streptomyces transformants. By resuspending mycelium into 2.5-10% dimethyl sulfoxide (DMSO), extensive manipulation prior to PCR could be avoided, thus significantly reducing screening time. Results proved to be both reliable and specific.
摘要
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