Torres C, Muñoz de Rueda P, Ruiz-Extremera A, Quintero D, Palacios A, Salmerón J
Digestive Service, Hospital Universitario San Cecilio, Avda. Dr. Olóriz, 16. 18012 Granada.
Rev Esp Enferm Dig. 2002 Nov;94(11):659-68.
To determine HCV and HGV replication sites in patients with chronic hepatitis C and to study interaction between these two viruses.
HGV RNA was studied in 272 patients with chronic hepatitis C. Of these, 35 were positive (group I). Twenty-three patients with chronic hepatitis C not co-infected with HGV were selected (group II).
Genomic and antigenomic chains of HCV were studied in both groups and those of HGV in group I in serum samples, peripheral blood mononuclear cells and liver tissue. In group I genomic chains of HCV and HGV were observed in 86 and 100%, respectively (ns), in serum samples (n = 35), and antigenomic chains in 17 and 23%, respectively (ns). In mononuclear cell samples (n = 15) 100% presented the genomic chain of HCV and 60% presented that of HGV (p < 0.05). Antigenomic chains were detected in 13 and 33%, respectively (ns). In liver tissue (n = 25) genomic chains were observed in 100 and 12%, respectively (p < 0.001); the antigenomic chain of HCV was detected in 76% while that of HGV was not present (p < 0.001). In group II genomic chains of HCV were found to be present in a very high percentage in all samples, while antigenomic chains appeared in 13% of serum and mononuclear cell samples and 89% of liver samples.
HCV and HGV have different sites of replication: whereas HCV replicates mainly in the liver, HGV is not hepatotropic. Mononuclear cells could represent a replication site for HGV but they are less important for HCV. Lastly, HGV does not modify the viral replication of HCV.
确定慢性丙型肝炎患者中丙型肝炎病毒（HCV）和庚型肝炎病毒（HGV）的复制位点，并研究这两种病毒之间的相互作用。
对272例慢性丙型肝炎患者进行了HGV RNA检测。其中35例呈阳性（第一组）。选取23例未合并感染HGV的慢性丙型肝炎患者（第二组）。
对两组患者的血清样本、外周血单核细胞和肝组织进行了HCV基因组链和反基因组链以及第一组患者HGV基因组链和反基因组链的研究。在第一组血清样本（n = 35）中，HCV基因组链和HGV基因组链的检出率分别为86%和100%（无显著性差异），反基因组链的检出率分别为17%和23%（无显著性差异）。在单核细胞样本（n = 15）中，100%的样本检测到HCV基因组链，60%的样本检测到HGV基因组链（p < 0.05）。反基因组链的检出率分别为13%和33%（无显著性差异）。在肝组织样本（n = 25）中，HCV基因组链和HGV基因组链的检出率分别为100%和12%（p < 0.001）；76%的肝组织样本检测到HCV反基因组链，未检测到HGV反基因组链（p < 0.001）。在第二组中，所有样本中HCV基因组链的检出率都非常高，而血清和单核细胞样本中反基因组链的检出率为13%，肝组织样本中为89%。
HCV和HGV具有不同的复制位点：HCV主要在肝脏中复制，而HGV并非嗜肝性病毒。单核细胞可能是HGV的复制位点，但对HCV而言其重要性较低。最后，HGV不会改变HCV的病毒复制。
